Uncovering the mechanisms by which light sensors enhance the freezing tolerance of plants
| Project/Area Number |
25850073
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 低温応答 / 葉緑体 / 植物 / 光 |
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| Outline of Final Research Achievements |
This project aimed at uncovering the mechanisms by which chloroplasts activate low-temperature signaling pathway as light sensors in plants. We fused the promoter region of COR15A to luciferase gene, and introduced this chimeric gene into Arabidopsis. This allowed us to visualize low temperature response based on bioluminescence derived from luciferase. We were able to establish the system that can screen low molecular mass compounds affecting low temperature response in plants. Furthermore, we developed a method of recovering seeds from the seedling lethal Arabidopsis mutant ppi2-2, allowing us to analyze the roles of developed chloroplasts in low temperature response.
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Report
(3 results)
Research Products
(4 results)
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[Journal Article] Targeting of a polytopic membrane protein to the inner envelope membrane of chloroplasts in vivo involves multiple transmembrane segments.2014
Author(s)
Okawa, K., Inoue, H., Adachi, F., Nakayama, K., Ito-Inaba, Y., Schnell, D.J., Uehara, S. and Inaba, T.
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Journal Title
J. Exp. Bot.
Volume: 65
Issue: 18
Pages: 5257-5265
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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