Molecular mechanism of mitochondrial proteins using ubiquinone as a cofactor
| Project/Area Number |
25850081
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
MURAI Masatoshi 京都大学, (連合)農学研究科(研究院), 助教 (80543925)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAMUKAI Makoto 島根大学, 大学生物資源科学部, 教授 (70186138)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ユビキノン / Coq10 / 光親和性標識 / ミトコンドリア / 複合体-I / Click Chemistry / ユビキノン結合性タンパク質 |
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| Outline of Final Research Achievements |
Ubiquinone (UQ) is thought to diffuse freely in the inner mitochondrial membrane to facilitate electron transfer between the respiratory complexes. Recent studies have focused on physiological importance of mitochondrial Coq10, which is a member of the steroidogenic acute regulatory protein (StAR)-related lipid transfer (START) domain superfamily. To get insights into the role of Coq10, we expressed the recombinant Schizosaccharomyces pombe Coq10 in Escherichia coli membrane, and carried out photoaffinity labeling study using a synthetic UQ probe. Comprehensive biochemical and proteomic analyses revealed that the UQ probe specifically binds to the N-terminus F39-K45 of the Coq10, which corresponds to the ligand-binding pocket in many proteins containing START domain. Our results provide the first exact evidence indicating that Coq10 accommodates UQ in a similar manner with other SATRT domain proteins do.
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Report
(3 results)
Research Products
(24 results)
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[Presentation] Site-Specific Chemical Modification of Mitochondrial NADH-Ubiquinone Oxidoreductase (Complex I) Through Ligand-Directed Tosylate Chemistry2014
Author(s)
Murai, M., Masuya, T., Morisaka, H., Ifuku, K., and Miyoshi, H.
Organizer
18th European Bioenergetics Conference (EBEC2014)
Place of Presentation
Universidade de Lisboa, Lisbon, Portugal
Year and Date
2014-07-12
Related Report
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