| Project/Area Number |
25850082
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | ペプチド / 細胞膜透過ペプチド / ヒスチジン / ゴルジ体 / リソソーム / 腫瘍 / 薬物輸送システム / DDS / ヒト線維肉腫細胞 / ヒト扁平上皮癌細胞 / マクロピノサイトーシス |
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| Outline of Final Research Achievements |
Cell-penetrating peptides (CPPs) are arginine/lysine-rich sequences, and they are effectively internalized into cells. On the other hand, in the present study, we found that polyhistidine peptide (H16: HHHHHHHHHHHHHHHH-NH2) was efficiently internalized into the HT1080 human fibrosarcoma cells. Cellular uptake of the H16 peptide was mainly due to micropinocytosis. Although most of the H16 peptide localized in the lysosome and Golgi apparatus, a proportion of the H16 peptide escaped from the macropinosome to the cytoplasm. In a protein transduction study, green fluorescence protein fused to the H16 peptide (GFP-H16) was also internalized into cells. Furthermore, in vivo study showed that the H16 peptide accumulated immediately in tumor tissue and showed long residence-time in the tumor (HT1080 human fibrosarcoma)-bearing mice. The findings suggest that the H16 peptide is novel CPP and represents a promising drug delivery carrier candidate in medical and biotechnological fields.
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