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Online evaluation of superoxide radical scavenging activity of vegetables fat-soluble components as studied by HPLC-ESR spin-trapping measurements

Research Project

Project/Area Number 25850089
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Food science
Research InstitutionKyoto Institute of Technology

Principal Investigator

YAMAGUCHI Tomoko  京都工芸繊維大学, ベンチャーラボラトリー, 研究員 (90644914)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords流通系ESR / HPLC-ESR / 抗酸化活性 / スーパーオキシドアニオンラジカル / コーヒー酸換算値 / FI-ESR / 青果物 / ガウス線形シミュレーション / 抗酸化 / ESR / 脂溶性成分
Outline of Final Research Achievements

Recently, we have developed flow-injection-ESR and HPLC-ESR systems special for quantitative spin-trapping ESR detection of the superoxide radical by using DMPO, as a spin-trapping reagent. The flow-ESR techniques were employed to explore potent superoxide radical scavengers involved in foods. In the present study, HPLC-ESR measurements were made for water-soluble components of foods. The determination of antioxidant activity was calculated using ESR line simulation.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2015 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) Remarks (2 results)

  • [Journal Article] Application of a Flow-injection Spin-trapping ESR Method for Evaluating the Alkoxy Radical Elimination Capacity (AREC) of Selected Antioxidants2015

    • Author(s)
      Yasuhiro Ssakurai, Tomoko Yamaguchi, Akira Nakajima, Kenji Kanaori, and Kunihiko Tajima
    • Journal Title

      Chemistry Letters

      Volume: 44 Issue: 6 Pages: 752-754

    • DOI

      10.1246/cl.150121

    • NAID

      130005074599

    • ISSN
      0366-7022, 1348-0715
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Quantitative Spin-trapping ESR Investigation of Alkoxyl Radical Derived from AAPH: Development of a Flow-injection Spin-trapping ESR System for the Oxygen Radical Absorbance Capacity Assay2015

    • Author(s)
      Akira Nakajima,Tomoko Yamaguchi,Tomoyuki Yamashita,Kiyoshi Kawai,Yusuke Miyake,Kenji Kanaori,Kunihiko Tajima
    • Journal Title

      Applied Magnetic Resonance

      Volume: 46 Issue: 9 Pages: 1-10

    • DOI

      10.1007/s00723-015-0664-5

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a Flow-injection Spin-trapping ESR System for Estimation of Second-order Rate Constants for Reactions of a Superoxide Radical Anion and Selected Phenolic Compounds2014

    • Author(s)
      Yasuhiro Sakurai, Tomoko Yamaguchi, Akira Nakajima, Kenji Kanaori, and Kunihiko Tajima
    • Journal Title

      Chemistry letters

      Volume: 43 Pages: 527-529

    • NAID

      130004868069

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] HPLC-ESR法によるカラム溶出成分のO2-・消去活性評価方法2015

    • Author(s)
      山口智子, 田中宏治, 三宅祐輔, 金折賢二, 田嶋邦彦
    • Organizer
      日本化学会 第95春季年会
    • Place of Presentation
      日本大学 理工学部船橋キャンパス
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Superoxide radical scavenging activity of cooked rice as studied by HPLC-ESR spin-trapping measurements2014

    • Author(s)
      Tomoko Yamaguchi , Gen Httori, Kenji Kanaori, Kunihiko Tajima, Isamu Tachimoto
    • Organizer
      APES-IES-SEST 2014
    • Place of Presentation
      Todaiji culture center
    • Year and Date
      2014-11-12 – 2014-11-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] HPLC-ESR法による炊飯米の水溶性成分のO2-・消去活性評価2014

    • Author(s)
      山口 智子, 服部 玄, 金折 賢二, 田嶋 邦彦, 舘本 勲武
    • Organizer
      61回日本食品科学工学会
    • Place of Presentation
      中村学園大学
    • Year and Date
      2014-08-28 – 2014-08-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] HPLC-ESR法による炊飯米水溶性成分のO2-・消去活性評価2014

    • Author(s)
      山口 智子, 服部 玄, 金折 賢二, 田嶋 邦彦, 舘本 勲武
    • Organizer
      第18回ESRフォーラム
    • Place of Presentation
      名古屋大学シンポジオン会議室
    • Year and Date
      2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] HPLC-ESR法によるタケノコの水溶性成分に含まれるO2-・消去活性物質の探索と活性評価2013

    • Author(s)
      山口 智子、田中 宏治、櫻井 康博、金折 賢二, 田嶋 邦彦
    • Organizer
      第17回ESRフォーラム
    • Place of Presentation
      九州大学医学部百年講堂
    • Related Report
      2013 Research-status Report
  • [Presentation] 青果物におけるフリーラジカル消去活性評価および成分評価2013

    • Author(s)
      服部 玄、山口 智子、田中 宏治、櫻井 康博、徳田 尚美、田嶋 邦彦、丹羽 真清
    • Organizer
      第66回日本酸化ストレス学会 学術集会
    • Place of Presentation
      名古屋市ウインク愛知
    • Related Report
      2013 Research-status Report
  • [Presentation] 青果物におけるフリーラジカル消去活性評価および成分評価2013

    • Author(s)
      服部 玄、山口 智子、田中 宏治、櫻井 康博、徳田 尚美、田嶋 邦彦、丹羽 真清
    • Organizer
      第 60 回記念大会 日本食品科学工学会
    • Place of Presentation
      実践女子大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 流通型ESR法による青果物水溶性のスーパーオキシドラジカル消去活性の解析2013

    • Author(s)
      田嶋 邦彦、山口 智子、田中 宏治、櫻井 康博、金折 賢治、服部 玄
    • Organizer
      第52回電子スピンサイエンス学会
    • Place of Presentation
      大宮ソニックシティ
    • Related Report
      2013 Research-status Report
  • [Remarks] デリカフーズ株式会社ホームページ

    • URL

      http://www.delica.co.jp/press/index_26.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 京都工芸繊維大学 ベンチャーラボラトリー ホームページ

    • URL

      http://www.vl.kit.jp/ja/project/project_01.html

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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