Studies on injury, viable but non-culturable state, and recovery of Campylobacter jejuni
Project/Area Number |
25850093
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Food science
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Research Institution | Fukuoka Women's University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | Campylobacter jejuni / heat injury / recovery / Campylobacter / 加熱損傷 / 回復 / カンピロバクター / VNC |
Outline of Final Research Achievements |
Heat injury and recovery from heat injury of Campylobacter jejuni was investigated. Heat injured C. jejuni cells were reproducibly generated by heating cell suspension (in PBS) of C. jejuni at 50°C for 5 minutes. Healthy cells retained culturability both on mCCDA and nutrient agar supplemented with 0.3% NaCl (NA). On the other hand, heat injured cells formed colonies only on mCCDA. Heat injured cells recovered cultivability on NA supplemented with 0.25 g/L sodium pyruvate. The results suggested that glycolysis of C. jejuni had been damaged by non-lethal heating. And the addition of sodium pyruvate would be important for the reliable detection of C. jejuni from foods and environments.
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Report
(3 results)
Research Products
(1 results)