Studies on injury, viable but non-culturable state, and recovery of Campylobacter jejuni

Research Project

Project/Area Number	25850093
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Food science
Research Institution	Fukuoka Women's University
Principal Investigator	KOBAYASHI Hiroshi 福岡女子大学, 文理学部, 講師 (00610255)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	Campylobacter jejuni / heat injury / recovery / Campylobacter / 加熱損傷 / 回復 / カンピロバクター / VNC
Outline of Final Research Achievements	Heat injury and recovery from heat injury of Campylobacter jejuni was investigated. Heat injured C. jejuni cells were reproducibly generated by heating cell suspension (in PBS) of C. jejuni at 50°C for 5 minutes. Healthy cells retained culturability both on mCCDA and nutrient agar supplemented with 0.3% NaCl (NA). On the other hand, heat injured cells formed colonies only on mCCDA. Heat injured cells recovered cultivability on NA supplemented with 0.25 g/L sodium pyruvate. The results suggested that glycolysis of C. jejuni had been damaged by non-lethal heating. And the addition of sodium pyruvate would be important for the reliable detection of C. jejuni from foods and environments.

Report

(3 results)