| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
From a lipophilic fraction of Eisenia arborea extract, bis(2-ethylhexyl) phthalate was isolated and idetified as an active compound. Four brown algal extracts and 2 purified phlorotannins had permeated Caco-2 cells, and the permeation ratios were 3-17 % and about 30 %, respectively. Condenced phlorotannin (Pt) excerted anti-allergic effect on pollen allergy and food allergy model mice. The mechanism would attribute to immunomodulating activity of Pt. To investigate anti-degranulation effect of Pt on mast cells, the effect of it on expression of protein kinase Cα (PKCα) in RBL-2H3 cells was measured by western blot method. But, the suppression of Pt on PKCα protein expression was not observed. Moerover, in vivo and in vitro experiments, fucoxanthin that is a marine carotenoid indicated anti-allergic effects.
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