Development of a method for rapidly detecting and identifying noxious microorganisms

Research Project

Project/Area Number	25850100
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Food science
Research Institution	Kumamoto Industrial Research Institute
Principal Investigator	SATOH TAKAO 熊本県産業技術センター(ものづくり室、材料・地域資源室、食品加工室), その他部局等, 研究主任 (80467977)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	食品安全性 / 質量分析 / 微生物 / 高速液体クロマトグラフ / 微生物同定
Outline of Final Research Achievements	We established a method for rapidly detecting and identifying noxious microorganisms using high performance liquid chromatograph mass spectrography and capillary electrophoresis mass spectrography. We estimated the measured accurate mass of the microbial metabolite using LC/MS or CE/MS system. As a result, we succeeded in identifying noxious microorganisms without isolating microorganisms and PCR.