Construction of basis of molecular breeding for male sterile trees in Cryptomeria japonica and development of selection markers

• Project/Area Number: 25850116
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Forest science
• Research Institution: Niigata University
• Principal Investigator: Moriguchi Yoshinari 新潟大学, 自然科学系, 助教 (60644804)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 連鎖解析 / 無花粉スギ / 連鎖地図 / マーカー選抜

Outline of Final Research Achievements

Using information from this high-density map of Cryptomeria japonica, we newly located three male-sterile genes (ms2, ms3 and ms4) on the 5th, 1st and 4th linkage groups, respectively, and constructed partial linkage maps around these loci. In addition, we have constructed partial linkage maps surrounding a male sterility gene (ms1) in four families (TO13S, S3T67, F1N4 and T5). In all four families, the accuracy with which male-sterile trees could be identified using the closest markers was more than 96.0 %. We also developed an allele-specific PCR marker for identifying male-sterile trees in the TO13S family from which male-sterile seedlings are produced.

Research Products

• [Journal Article] A simple allele-specific PCR marker for identifying male-sterile trees: Towards DNA marker-assisted selection in the Cryptomeria japonica breeding program (2014)
  • Author(s): Moriguchi Y, Ueno S, Saito M, Higuchi Y, Miyajima D, Itoo S, Tsumura Y
  • Journal Title: Molecular Breeding Volume: 10 Issue: 4 Pages: 1069-1077
  • DOI: 10.1007/s11295-014-0743-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Establishment of a microsatellite panel covering the sugi (Cryptomeria japonica) genome, and its application for localization of a male sterile gene (ms-2). (2014)
  • Author(s): Moriguchi Y, Ueno S, Higuchi Y, Miyajima D, Itoo S, Futamura N, Tsumura Y
  • Journal Title: Molecular Breeding Volume: 33 Issue: 2 Pages: 315-325
  • DOI: 10.1007/s11032-013-9951-8
  • Peer Reviewed
• [Presentation] Sugi genome resources (2015)
  • Author(s): Ueno S, Moriguchi Y, Uchiyama K, Ujino-Ihara T, Futamura N, Matsumoto A, Tsumura Y
  • Organizer: ProCpGen Conifer Genomics Conference
  • Place of Presentation: オルレアン フランス
  • Year and Date: 2015-11-30
  • Int'l Joint Research
• [Presentation] スギ雄性不稔遺伝子（ms-1～ms-4）の連鎖地図上の位置の特定 (2015)
  • Author(s): 森口喜成、内山憲太郎、上野真義、伊原徳子、松本麻子、斎藤真己、岩井淳治、宮嶋大介、樋口有未、伊藤信治、佐藤雅哉、津村義彦
  • Organizer: 第128回日本育種学会
  • Place of Presentation: 新潟大学 新潟県新潟市
  • Year and Date: 2015-09-12
• [Presentation] 雄性不稔遺伝子ms-4のマッピング (2014)
  • Author(s): 佐藤雅哉、森口喜成、松本麻子、岩井淳治、宮嶋大介、津村義彦
  • Organizer: 第3回森林遺伝学会
  • Place of Presentation: 東京大学
  • Year and Date: 2014-11-07
• [Presentation] スギ高密度連鎖地図情報を利用した雄性不稔遺伝子と連鎖するDNAマーカーの開発
  • Author(s): 森口喜成・伊原徳子・内山憲太郎・上野真義・松本麻子・二村典宏・斎藤真己・樋口有未・伊藤信治・宮嶋大介・篠原健司・津村義彦
  • Organizer: 第124回日本育種学会
  • Place of Presentation: 鹿児島大学
• [Presentation] DNAマーカーを用いて芽生えの段階で無花粉スギの家系内選抜を簡単に行う手法の開発
  • Author(s): 森口喜成・上野真義・斎藤真己・樋口有未・宮嶋大介・伊藤信治・津村義彦
  • Organizer: 第2回森林遺伝学会
  • Place of Presentation: 東京大学
• [Presentation] スギ高密度基盤連鎖地図を利用した雄性不稔遺伝子（ms-3）のマッピング
  • Author(s): 森口喜成・上野真義・松本麻子・伊原徳子・岩井淳治・宮嶋大介・津村義彦
  • Organizer: 第125回日本森林学会
  • Place of Presentation: 大宮ソニックシティ