Study of xylem cell wall formation using isotopic imaging

• Project/Area Number: 25850120
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Wood science
• Research Institution: The University of Tokyo
• Principal Investigator: TAKEUCHI Miyuki 東京大学, 農学生命科学研究科, 特任助教 (20378912)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 細胞壁 / 安定同位体 / 二次イオン質量分析法 / 元素イメージング

Outline of Final Research Achievements

Stable isotopic labeling combined with isotopic imaging using SIMS was used in order to investigate process of carbon accumulation in wood. This study aimed to understand correlation between process of wood formation and carbon assimilation of photosynthesis. 13C-labelled carbon dioxide was applied to a poplar tree and distribution of the tracer 13C was analyzed. Time course of carbon accumulation to xylem cell wall was studied and it revealed change of 13C distribution in cell wall in subcellular scale. A part of carbon derived from CO2 was directly incorporated into xylem cell wall. Remarkable accumulation of the tracer 13C was detected in a narrow layer in the cell wall of the developing xylem.

Research Products

• [Journal Article] Elemental imaging of biological samples with high lateral resolution secondary ion mass spectrometry (2014)
  • Author(s): 竹内美由紀
  • Journal Title: PLANT MORPHOLOGY Volume: 26 Issue: 1 Pages: 19-23
  • DOI: 10.5685/plmorphol.26.19
  • NAID: 130005066542
  • ISSN: 0918-9726, 1884-4154
  • Peer Reviewed
• [Presentation] Isotopic imaging of carbon accumulation during xylem cell wall formation using 13CO2 pulse labeling (2015)
  • Author(s): Miyuki Takeuchi, Mariko Norisada and Akira Isogai
  • Organizer: IAWPS2015 International Symposium on Wood Science and Technology
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 2015-03-15 – 2015-03-17
• [Presentation] SIMSを用いた植物体内における炭素移動の観察 (2014)
  • Author(s): 竹内美由紀、磯貝明
  • Organizer: 平成26年度 生理学研究所研究会 電子顕微鏡機能イメージングの医学・生物学への応用
  • Place of Presentation: 自然科学研究機構 岡崎コンファレンスセンター，岡崎
  • Year and Date: 2014-11-12 – 2014-11-13
  • Invited
• [Presentation] 同位体イメージングによる植物細胞壁への光合成産物堆積過程の解析 (2014)
  • Author(s): 竹内美由紀、磯貝明
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学 生田キャンパス、川崎
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] Visualizing accumulation of photosynthetic products in plant using isotopic labeling and SIMS (2014)
  • Author(s): Miyuki Takeuchi, Akira Isogai
  • Organizer: SISS16 The Scientific International Symposium on SIMS and Related Techniques Based on Ion-Solid Interactions
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2014-06-19 – 2014-06-20
  • Invited
• [Presentation] 二次イオン質量分析法を用いた同位体イメージングによる 光合成産物の木部細胞壁への堆積過程の解析 (2014)
  • Author(s): 竹内美由紀、磯貝明
  • Organizer: 第64回 日本木材学会
  • Place of Presentation: 愛媛大学城北キャンパス
• [Presentation] 二次イオン質量分析法による樹木内の炭素移動の可視化 (2013)
  • Author(s): 竹内美由紀、磯貝明
  • Organizer: 日本植物学会第77回大会
  • Place of Presentation: 北海道大学札幌キャンパス
  • Invited
• [Presentation] Imaging of carbon incorporation in tree using 13CO2 pulse labeling and NanoSIMS (2013)
  • Author(s): Miyuki Takeuchi, Akira Isogai
  • Organizer: 19th International Conference on Secondary Ion Mass Spectrometry
  • Place of Presentation: Jungmun Sightseeing Complex, Seogwipo City, Jeju-do, Korea