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Degradation of regenerated cellulose treated with ionic liquids by monocomponent cellulases

Research Project

Project/Area Number 25850122
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Wood science
Research InstitutionShinshu University

Principal Investigator

MIZUNO Masahiro  信州大学, 学術研究院工学系, 助教 (60432168)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsセルラーゼ / イオン液体 / 再生セルロース / 酵素分解
Outline of Final Research Achievements

Ionic liquid (IL) is a salt composed of an organic cation and a variety of organic and inorganic anions, and some of them can dissolve cellulose under mild condition without any other compound. After dissolution of cellulose in IL, cellulose is easily regenerated by the addition of an anti-solvent such as water. For the effective hydrolysis of cellulose treated with IL, it is necessary to prepare the cellulase mixture containing an adequate ration of cellulase component according to crystal allomorph and the crystallinity of regenerated cellulose. Here, the reactivity of each monocomponent cellulase for the regenerated cellulose is investigated. As a result, ILs that provide cellulose II type regenerated cellulose were better suited for enzymatic degradation. Furthermore, endoglucanase was more effective for the degradation of regenerated cellulose rather than exo-type cellulase.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2014 2013

All Presentation (3 results)

  • [Presentation] イオン液体処理セルロースのモノコンポーネント酵素を用いた糖化性の評価2014

    • Author(s)
      水野正浩、吉崎涼、野﨑功一、天野良彦
    • Organizer
      セルロース学会第21回年次大会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県・鹿児島市)
    • Year and Date
      2014-07-17 – 2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Enzymatic hydrolysis of cellulose regenerated from ionic liquid pretreatment using monocomponent cellulase2013

    • Author(s)
      Masahiro Mizuno, Kouichi Nozak, Toshiyuki Itoh, Yoshihiko Amano
    • Organizer
      The 17th International Symposium on Wood, Fiber and Pulping Chemistry
    • Place of Presentation
      Sheraton Vancouver Wall Center, Canada
    • Related Report
      2013 Research-status Report
  • [Presentation] 水生植物に対するイオン液体処理の効果及び酵素糖化性の評価2013

    • Author(s)
      水野正浩,古邸洋紀,佐藤伸明,野﨑功一,天野良彦
    • Organizer
      セルロース学会第20回年次大会
    • Place of Presentation
      京都大学宇治キャンパス
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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