| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The secondary cell wall is divided into three layers based on the orientation of cellulose microfibrils (MFs). The alignment of MFs is guided by cortical microtubules (CMTs) and its spatial distribution is rearranged during the developmental process of xylem cells. The aim of this study is to reveal intracellular mechanisms that regulate spatial pattern of CMTs in xylem fibers. First, we tried to visualize CMTs in fibers using the transgenic poplars expressing GFP-tagged α- and β-tubulin. However, GFP fluorescence representing CMTs was not imaged in almost all cells. Second, we attempted to identify transcription factors that control spatial pattern of CMTs in xylem cells. The distribution of CMTs was more aligned in the transgenic poplar overexpressing TF26 and TF34. Since the genes were preferentially expressed in secondary xylem and regulated microtubule-associated proteins as downstream targets, they may have a key role in ordered alignments of CMTs in secondary xylem cells.
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