Studies of oncogenesis and viral pathogenesis of Oncorhunchus masou virus (OMV; Salmonid herpesvirus 2) in salmonid fish

• Project/Area Number: 25850132
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science
• Research Institution: Hokkaido University
• Principal Investigator: Kasai Hisae 北海道大学, 水産科学研究科(研究院), 准教授 (50399995)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: OMV / 魚類ウイルス / ヘルペスウイルス / 腫瘍

Outline of Final Research Achievements

Oncorhynchus masou virus (OMV; Salmonid herpesvirus 2) is a member of Herpisviridae associated with oncogenic and hepatitic diseases of national and international socio-economic importance. The present study focused on oncogenesis and viral pathogenesis of OMV in salmonid fish. Viral genes were localized in the whole Tumor tissues of masu salmon Oncorhynchus masou that survived OMV infections. A strong ISH signal was detected in the tumor tissue indicating that OMV induces tumor development. Highly expressed viral genes in Tumor were found by transcriptome analysis using NGS-based RNA sequencing. Liver failure as well as renal dysfunction and failure of gill function were occurred in OMV-infected rainbow trout O. mykiss.

Research Products

• [Presentation] Oncorhynchus masou virus (OMV) 感染耐過サクラマス親魚の腫瘍および脳組織におけるウイルス潜伏部位 (2016)
  • Author(s): 池本恵祐・勝又義友・都木靖彰・吉水 守・笠井久会
  • Organizer: 平成28年度日本魚病学会春季大会
  • Place of Presentation: 日本獣医生命大学（東京都武蔵野市）
  • Year and Date: 2016-03-12
• [Presentation] 魚類ウイルス病とその防除対策 (2015)
  • Author(s): 笠井久会
  • Organizer: 日本ウイルス学会北海道支部夏季シンポジウム
  • Place of Presentation: 大滝セミナーハウス（北海道伊達市）
  • Year and Date: 2015-07-25
  • Invited
• [Presentation] Oncorhynchus masou virus (OMV) の感染部位 (2015)
  • Author(s): 池本恵祐・勝又義友・笠井久会
  • Organizer: 平成27年度日本魚病学会春季大会
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2015-03-07 – 2015-03-08
• [Presentation] Oncorhynchus masou virus (OMV) のサケ科魚類に対する病原性 (2015)
  • Author(s): 池本恵祐・笠井久会・吉水 守・降幡 充・小原昌和
  • Organizer: 平成27年度日本魚病学会春季大会
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2015-03-07 – 2015-03-08
• [Presentation] サケ科魚ヘルペスウイルスOncorhynchus masou virus (OMV) の全ゲノム解析 (2013)
  • Author(s): 笠井久会・吉水 守
  • Organizer: 第7回サケ学研究会
  • Place of Presentation: 北海道大学札幌キャンパス（北海道札幌市）
• [Book] 魚介類の微生物感染症の治療と予防 (2013)
  • Author(s): 笠井久会
  • Total Pages: 504
  • Publisher: 恒星社厚生閣