Monitoring of pesticide residues in farmed fish by bioluminescent reporter gene assay
| Project/Area Number |
25850135
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | ルシフェラーゼ / Two-hybrid system / ティラピア / EPC細胞 / AhR / PXR / CYP1B1 / ヘテロ二量体 / ツーハイブリッドシステム / タンパク質間相互作用 / 転写活性 / 薬物代謝 / 農薬 / 細胞 |
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| Outline of Final Research Achievements |
Pesticides have been prohibited to use for aquaculture, but still detected from farmed fish. Although pesticide residues in fish are monitored by GC/MS or LC/MS, these are not used routinely in most fish farming due to their high cost operation and time requirement for sample preparation.
CYPs and P-gp play important roles in drug metabolism. The transcriptions of CYPs and P-gp are regulated by drug receptors such as AhR and PXR. Here, we investigated whether the cell that contains these molecules in control of a reporter gene is applicable to monitoring of pesticide residues in fish.
The heterodimerization between AhR and Arnt was investigated by two-hybrid system on fish cells. AhR forms a heterodimer with Arnt in the presence of pesticides. Moreover, the expression of CYP1B1 in hepatocytes was induced by pesticides. These results suggest that AhR/Arnt heterodimerization becomes reliable cellular reporter for monitoring the pesticide residues in fish.
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Report
(4 results)
Research Products
(4 results)
