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Monitoring of pesticide residues in farmed fish by bioluminescent reporter gene assay

Research Project

Project/Area Number 25850135
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Aquatic bioproduction science
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

FUTAMI Kunihiko  東京海洋大学, その他部局等, 助教 (00513459)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsルシフェラーゼ / Two-hybrid system / ティラピア / EPC細胞 / AhR / PXR / CYP1B1 / ヘテロ二量体 / ツーハイブリッドシステム / タンパク質間相互作用 / 転写活性 / 薬物代謝 / 農薬 / 細胞
Outline of Final Research Achievements

Pesticides have been prohibited to use for aquaculture, but still detected from farmed fish. Although pesticide residues in fish are monitored by GC/MS or LC/MS, these are not used routinely in most fish farming due to their high cost operation and time requirement for sample preparation.
CYPs and P-gp play important roles in drug metabolism. The transcriptions of CYPs and P-gp are regulated by drug receptors such as AhR and PXR. Here, we investigated whether the cell that contains these molecules in control of a reporter gene is applicable to monitoring of pesticide residues in fish.
The heterodimerization between AhR and Arnt was investigated by two-hybrid system on fish cells. AhR forms a heterodimer with Arnt in the presence of pesticides. Moreover, the expression of CYP1B1 in hepatocytes was induced by pesticides. These results suggest that AhR/Arnt heterodimerization becomes reliable cellular reporter for monitoring the pesticide residues in fish.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2015 2014

All Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Presentation] Aryl hydrocarbon receptor-mediated gene expression in Nile tilapia Oreochromis niloticus exposed to pesticides2015

    • Author(s)
      Kunihiko Futami, Kano Matsunaga, Takayuki Katagiri, Makoto Endo and Masashi Maita
    • Organizer
      NRCT-JSPS Asian Core Program Symposium 2016
    • Place of Presentation
      Kasetsart University, Bangkok, Thailand
    • Year and Date
      2015-12-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Drug receptor-mediated gene expression in Nile tilapia Oreochromis niloticus exposed to pesticides2015

    • Author(s)
      Kunihiko Futami
    • Organizer
      AQUATIC PRODUCTS PROCESSING: Cleaner Production Chain for Healthier Food
    • Place of Presentation
      Can Tho University, Vietnum
    • Year and Date
      2015-12-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マラカイトグリーンによるティラピアPXR/RXRの転写活性の阻害2014

    • Author(s)
      木下祥二郎・松永花野・片桐孝之・舞田正志・延東真・二見邦彦
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] マラカイトグリーンはティラピアPXRとRXRのタンパク質間相互作用を阻害する2014

    • Author(s)
      木下祥二郎・松永花野・片桐孝之・舞田正志・延東真・二見邦彦
    • Organizer
      平成26年度日本魚病学会秋季大会
    • Place of Presentation
      九州大学中央図書館視聴覚ホール
    • Year and Date
      2014-09-22 – 2014-09-23
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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