| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Transcriptome data of Kudoa septempunctata (Ks) was obtained by next-generation sequencing. EF1-α and Hsp83 of Ks shared the highest identity to EF1-α of threadworm and Hsp90 of flatworm, respectively. The enzymatic activities were investigated using APIZYM. Positive signals were observed for leucine arylamidase, acid phosphatase and naphthol-AS-BI-phosphohydrolase.
The production of a monoclonal antibody was attempted. Mice were injected with an emulsion containing spores. The fusion of lymphocytes with myeloma cells produced 48 hybridoma lines, of which two were selected on the basis of their reactivity.
The sequencing analysis of mitochondrial DNA revealed the presence of genetic polymorphisms of Ks. Four genotypes were identified based on a PCR-RFLP assay. The genotyping analysis of 32 specimens were revealed that all genotypes were found in domestic samples, and appearance frequency of each genotype were regionally uneven, whereas only genotype II was found in oversea sample.
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