Development of a genotyping assay and characterization of Kudoa septempunctata
Project/Area Number |
25850139
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Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Aquatic bioproduction science
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Research Institution | Japan Fisheries Research and Education Agency |
Principal Investigator |
Mekata Tohru 国立研究開発法人水産総合研究センター, 増養殖研究所, 研究員 (40597944)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | クドア・セプテンプンクタータ / モノクローナル抗体 / 酵素活性 / 遺伝子型別 / クドア / 遺伝子型 / 抗体 / 遺伝子解析 / 魚病 / ヒラメ |
Outline of Final Research Achievements |
Transcriptome data of Kudoa septempunctata (Ks) was obtained by next-generation sequencing. EF1-α and Hsp83 of Ks shared the highest identity to EF1-α of threadworm and Hsp90 of flatworm, respectively. The enzymatic activities were investigated using APIZYM. Positive signals were observed for leucine arylamidase, acid phosphatase and naphthol-AS-BI-phosphohydrolase. The production of a monoclonal antibody was attempted. Mice were injected with an emulsion containing spores. The fusion of lymphocytes with myeloma cells produced 48 hybridoma lines, of which two were selected on the basis of their reactivity. The sequencing analysis of mitochondrial DNA revealed the presence of genetic polymorphisms of Ks. Four genotypes were identified based on a PCR-RFLP assay. The genotyping analysis of 32 specimens were revealed that all genotypes were found in domestic samples, and appearance frequency of each genotype were regionally uneven, whereas only genotype II was found in oversea sample.
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Report
(4 results)
Research Products
(6 results)