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Endocrine regulation of crustacean vitellogenesis: transcriptome and proteome analyses of the ovary

Research Project

Project/Area Number 25850143
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Aquatic life science
Research InstitutionOkayama University

Principal Investigator

TSUTSUI Naoaki  岡山大学, 理学部, 助教 (00643785)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords甲殻類 / 生理学 / 卵黄形成 / ホルモン / 内分泌 / クルマエビ / 増養殖 / ビテロジェニン
Outline of Final Research Achievements

Crustacean vitellogenesis is regulated by vitellogenesis-inhibiting hormone (VIH) from the X-organ/sinus gland neurosecretory system in the eyestalks. To investigate the mode of action of VIH in the ovary, transcriptome and proteome analyses were performed. Transcriptome analyses revealed that several hormonal factors presumed to be involved in the regulation of vitellogenesis or development of secondary sex characteristics, suggesting that peripheral tissues contribute to the regulation of ovarian development. In addition, sex-lethal, piwi, and pumilio, which were involved in the regulation of the renewal and differentiation of germline stem cell in Drosophila, were identified by proteome analyses.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013

All Journal Article (6 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (6 results)

  • [Journal Article] Seasonal variations in reproductive activity of the blue crab, Callinectes sapidus: Vitellogenin expression and levels of vitellogenin in the hemolymph during ovarian development.2015

    • Author(s)
      Willawan Thongda, J. Sook Chung, Naoaki Tsutsui, Nilli Zmora, Anna Katenta
    • Journal Title

      Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology

      Volume: 179 Pages: 35-43

    • DOI

      10.1016/j.cbpa.2014.08.019

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of crustacean hyperglycemic hormone-family peptides with vitellogenesis-inhibiting activity from the shiba shrimp Metapenaeus joyneri.2015

    • Author(s)
      Midori Fukushima, Sayaka Kotaka, Naoaki Tsutsui, Kiyoshi Asahina, Susumu Izumi, Tsuyoshi Ohira
    • Journal Title

      Fisheries Science

      Volume: 81 Issue: 1 Pages: 65-72

    • DOI

      10.1007/s12562-014-0834-7

    • NAID

      40020352329

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] クルマエビのビテロジェニン遺伝子発現に対する眼柄内ペプチドの影響2014

    • Author(s)
      筒井直昭
    • Journal Title

      日本水産学会誌

      Volume: 80 Pages: 531-531

    • NAID

      130004678133

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Direct evidence for the function of crustacean insulin-like androgenic gland factor (IAG): Total chemical synthesis of IAG2014

    • Author(s)
      Hidekazu Katayama, Nozomi Kubota, Hironobu Hojo, Ayumi Okada, Sayaka Kotaka, Naoaki Tsutsui, Tsuyoshi Ohira
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 22 Issue: 21 Pages: 5783

    • DOI

      10.1016/j.bmc.2014.09.031

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Dynamics of Vitellogenin and Vitellogenesis-Inhibiting Hormone Levels in Adult and Subadult Whiteleg Shrimp, Litopenaeus vannamei: Relation to Molting and Eyestalk Ablation.2014

    • Author(s)
      B. J. Kang, T. Okutsu, N. Tsutsui, J. Shinji, S.-H. Bae, M. N. Wilder.
    • Journal Title

      Biology of Reproduction

      Volume: 90 Issue: 1 Pages: 1-10

    • DOI

      10.1095/biolreprod.113.112243

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning of a cDNA encoding vitellogenesis-inhibiting hormone in the whiteleg shrimp Litopenaeus vannamei and preparation of its recombinant peptide using an E. coli expression system.2013

    • Author(s)
      N. Tsutsui, T. Ohira, T. Okutsu, J. Shinji, S.-H. Bae, B. J. Kang, M. N. Wilder.
    • Journal Title

      Fisheries Science

      Volume: 79 Issue: 3 Pages: 357-365

    • DOI

      10.1007/s12562-013-0603-z

    • NAID

      10031158682

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] クルマエビの卵巣で発現する甲殻類雌性ホルモン (CFSH) のcDNAクローニング2015

    • Author(s)
      甲高彩華, 筒井直昭, 大平剛
    • Organizer
      平成27年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(港区)
    • Year and Date
      2015-03-27 – 2015-03-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Chemical synthesis and function of insulin-like androgenic gland factor from the kuruma prawn Marsupenaeus japonicus.2014

    • Author(s)
      Hidekazu Katayama, Nozomi Kubota, Hironobu Hojo, Ayumi Okada, Sayaka Kotaka, Naoaki Tsutsui, Tsuyoshi Ohira
    • Organizer
      第39回日本比較内分泌学会大会
    • Place of Presentation
      岡崎コンファレンスセンター(岡崎市)
    • Year and Date
      2014-11-07 – 2014-11-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] An in vitro bioassay for an androgenic gland hormone activity using the ovarian culture of the kuruma prawn Marsupenaeus japonicus.2014

    • Author(s)
      Tsuyoshi Ohira, Ayumi Okada, Sayaka Kotaka, Naoaki Tsutsui, Nozomi Kubota, Hidekazu Katayama
    • Organizer
      第39回日本比較内分泌学会大会
    • Place of Presentation
      岡崎コンファレンスセンター(岡崎市)
    • Year and Date
      2014-11-07 – 2014-11-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 卵黄形成抑制ホルモンの作用機序解明に向けたクルマエビ卵巣の遺伝子の網羅的解析2014

    • Author(s)
      筒井直昭, 小林靖尚, 泉川晃一, 坂本竜哉
    • Organizer
      平成26年度日本水産学会秋季大会
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2014-09-19 – 2014-09-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 軟骨魚類アカエイの子宮内液に含まれるタンパク質の精製と解析2014

    • Author(s)
      筒井直昭 小林靖尚 坂本竜哉
    • Organizer
      平成26年度公益社団法人日本水産学会春季大会
    • Place of Presentation
      北海道大学函館キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 水田水域における外来種アメリカザリガニの駆除に用いる人工巣穴2013

    • Author(s)
      牛見悠奈 宮武優太 筒井直昭 中田和義
    • Organizer
      平成25年度農業農村工学会大会講演会
    • Place of Presentation
      東京農業大学
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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