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Development of evaluation/diagnosis technology for quality in fruits and vegetables based on the dysfunction of cell membrane

Research Project

Project/Area Number 25850172
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Agricultural environmental engineering/Agricultural information engineering
Research InstitutionKobe University

Principal Investigator

KUROKI Shinichiro  神戸大学, (連合)農学研究科(研究院), 助教 (00420505)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords可視近赤外分光法 / 脂質過酸化物 / 膜透過性 / 低酸素貯蔵 / 細胞膜水伝導係数 / 膜機能劣化 / 可視近赤外スペクトル / 水伝導係数 / 細胞膜 / 二層流法 / プロトプラスト / ポストハーベスト工学 / 非破壊品質評価
Outline of Final Research Achievements

The objectives of this study were to examine the changes in permeability and lipid peroxide of biomembrane in postharvest spinach leaves, and to develop nondestructive measurement technology for those properties using visible and near infrared spectroscopy. Results revealed that membrane lipid peroxide was gained prior to the increase in membrane permeability, suggesting that there is a threshold in the accumulation of lipid peroxide to increase membrane permeability. It was possible to construct calibration models having an R-square value above 0.5 for rate constant of ion leakage and malondialdehyde equivalent. This study gave the possibility of freshness evaluation of fruits and vegetables based on health assessment of the biomembrane using visible near-infrared spectroscopy.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (11 results)

All 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (10 results) (of which Invited: 3 results)

  • [Journal Article] Nondestructive freshness evaluation of spinach leaves under low oxygen storage using visible and near infrared spectroscopy2014

    • Author(s)
      Kuroki, S., Mizutani, R., Tachikawa, Y., Nakano, K.
    • Journal Title

      2013 IFAC Bio-Robotics Conference

      Volume: 1(1) Issue: 4 Pages: 302-306

    • DOI

      10.3182/20130327-3-jp-3017.00069

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Measurement and evaluation of dysfunction of cell membranes in fruits and vegetables during low oxygen storage2014

    • Author(s)
      Kuroki, S., Tachikawa, Y., Koizumi, K., Soga, A., Yoshida, M., Nakamura, N., Shiina, T., Nakano, K.
    • Organizer
      International Conference on Sustainable Agriculture, Food and Energy (SAFE2014)
    • Place of Presentation
      Dempasar, Indonesia
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低酸素貯蔵がホウレンソウ葉の細胞膜機能劣化に与える影響2014

    • Author(s)
      立川雄樹, 黒木信一郎, 小泉高陽, 曽我綾香, 吉田誠, 中村宣貴, 椎名武夫, 中野浩平
    • Organizer
      日本生物環境工学会2014年東京大会
    • Place of Presentation
      明治大学駿河台キャンパス
    • Year and Date
      2014-09-08 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細胞膜の物理化学的特性を指標とした青果物の品質評価法の開発2014

    • Author(s)
      黒木信一郎, 小泉高陽, 立川雄樹, 曽我綾香, 吉田誠, 中村宣貴, 椎名武夫, 中野浩平
    • Organizer
      第73回農業食料工学会年次大会
    • Place of Presentation
      琉球大学
    • Year and Date
      2014-05-16 – 2014-05-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低温貯蔵中におけるキュウリ果実内潜在ダメージの非破壊計測2014

    • Author(s)
      黒木信一郎, 小湊貴子, 伊藤博通, 中野浩平
    • Organizer
      第73回農業食料工学会年次大会
    • Place of Presentation
      琉球大学
    • Year and Date
      2014-05-16 – 2014-05-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新しい青果物品質評価論の創生への挑戦2014

    • Author(s)
      黒木信一郎
    • Organizer
      「作物と環境」研究会(ヤンマー株式会社)
    • Place of Presentation
      大阪
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Nondestructive measurements of biomembrane deterioration in fruits and vegetables using using VIS-NIR spectroscopy2014

    • Author(s)
      Kuroki, S.
    • Organizer
      Workshop on Innovation and Pioneering Technology (WINTech 2014)
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 低温障害による生体膜機能劣化とその非破壊計測技術開発2013

    • Author(s)
      黒木信一郎
    • Organizer
      農林水産消費安全技術センター(FAMIC)神戸センター/食の安全・安心科学センター合同発表会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Nondestructive measurements of biomembrane deterioration in cucumber fruit (Cucumis Sativa L.) during cold storage using VIS-NIR spectroscopy2013

    • Author(s)
      Kuroki, S., Kominato, T., Nakano, K.
    • Organizer
      ASEAN Regional Conference on Food Security
    • Place of Presentation
      Penang, Malaysia
    • Related Report
      2013 Research-status Report
  • [Presentation] ホウレンソウ葉内脂質過酸化レベルの非破壊モニタリング技術の開発2013

    • Author(s)
      立川雄樹, 黒木信一郎, 中野浩平
    • Organizer
      第72回農業食料工学会年次大会
    • Place of Presentation
      帯広
    • Related Report
      2013 Research-status Report
  • [Presentation] キュウリ果実の低温障害進行過程における生体膜機能劣化の非破壊計測2013

    • Author(s)
      小湊貴子, 黒木信一郎, 中野浩平
    • Organizer
      第72回農業食料工学会年次大会
    • Place of Presentation
      帯広
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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