| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Outline of Final Research Achievements |
The present study investigated the effect of mitochondrial reactive oxygen species (mtROS) production on protein degradation in skeletal muscle of birds exposed to heat stress. Heat stress resulted in simultaneous increases in mtROS production and ubiquitin ligase (atrogin-1) mRNA levels in the skeletal muscle tissues, which suggests an involvement of mtROS production in a transcription of atrogin-1 in these tissues. In avian muscle cells incubated in heat-stressed conditions, the simultaneous increases in mtROS and the ubiquitin ligase mRNA level were observed, and following the addition of Tempol into these cells not only mtROS production but also atrogin-1 mRNA level were restored to near-normal values. We here shows that mtROS production may play an important role in inducing protein degradation probably via an up-regulation of protein ubiquitination in avian skeletal muscle under heat stress conditions.
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