| Project/Area Number |
25850186
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | Saga University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 体外受精 / 暑熱ストレス / リソソーム / ウシ受精卵 / 胚発生 / ガラス化 / ウシ / 高温ストレス / 外的ストレス |
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| Outline of Final Research Achievements |
In this study, in order to obtain the fundamental information for elucidating the relationship of intracellular digestion and preimplantation embryo development in cattle, we clarified dynamics of cathepsin B activity and its expression, localization of lysosomes and autophagosomes. And also, our data show that the excess cathepsin B activity is induced under conditions of the environmental stress of culture. On the other hand, developmental competence of embryos exposed to culture stress can be improved by inhibition of cathepsin B activity. In conclusion, these findings implicate that cathepsin B activity as one of the possible pathways that are responsible for developmental arrest caused by culture stress. Moreover, regulating cathepsin B would be contributed for the improvement of in vitro embryo production system.
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