| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Adrenomedullin (AM) is a potent vasodilator peptide and is also involved in various physiological activities. The aim of this study is to determine the physiological role of AM in the regulation of bovine placental function during pregnancy. The results of present study demonstrate that secretion profile of AM in maternal plasma is almost consistent with AM gene expression pattern in the placenta, suggesting locally produced AM in bovine placenta is secreted into maternal circulation during pregnancy. The microarray analysis of bovine trophectoderm cells revealed that AM treatment up-regulates genes involved in lipid biosynthetic process, cell proliferation and apoptosis and down-regulates genes involved in cytoskeleton organization and cell adhesion. In addition, AM stimulated proliferation of both fetal and maternal placental cells. These findings suggest that AM may play a crucial role in the regulation of placental function on establishment and maintenance of bovine pregnancy.
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