Verification of ameliorating effect of lactic acid bacteria on atopic dermatitis based on the suppression of proinflammatory response of keratinocytes

• Project/Area Number: 25850190
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Animal production science
• Research Institution: Shinshu University
• Principal Investigator: KAWAHARA Takeshi 信州大学, 学術研究院農学系, 准教授 (30345764)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 乳酸菌 / 皮膚角化細胞 / アトピー性皮膚炎 / ケモカイン / TARC / TLR2

Outline of Final Research Achievements

This research aimed at evaluating the regulatory effect of lactobacillus strains on the proinflammatory responses in keratinocytes. Cell culture study showed that some Lactobacillus strains suppressed the expression of TARC (thymus and activation-regulated chemokine) in human keratinocyte cell line. Significant inhibitory effect on the development of dermatitis was observed by topical application of an extract obtained from disrupted bacterial body of the TARC-suppressive strain in atopic dermatitis model mice.
The study showed that a possibility of novel usage of a lactobacillus strains as a prevention material against the development of atopic dermatitis.

Research Products

• [Journal Article] Effect of Oral Administration of the IgE-suppressive Wild Yeast Strain <i>Saccharomyces paradoxus</i> P01 on the Development of Atopic Dermatitis-like Symptoms in NC/Nga Mice (2015)
  • Author(s): T. Kawahara, D. Nakayama, K. Tanaka, H. Yasui
  • Journal Title: Food Science and Technology Research Volume: 21 Issue: 2 Pages: 223-230
  • DOI: 10.3136/fstr.21.223
  • NAID: 130005098046
  • ISSN: 1344-6606, 1881-3984
  • Peer Reviewed
• [Journal Article] Inhibitory effect of a hot-water extract of leaves of Japanese big-leaf magnolia (Magnolia obovata) on rotavirus-induced diarrhea in mouse pups (2014)
  • Author(s): T. Kawahara, T. Tomono, Y. Hamauzu, K. Tanaka, H. Yasui
  • Journal Title: Evidence-Based Complementary and Alternative Medicine Volume: 365831 Issue: 1
  • DOI: 10.1155/2014/365831
  • NAID: 120007101330
  • Peer Reviewed / Open Access
• [Journal Article] Suppressive Effect of Wild <i>Saccharomyces cerevisiae</i> and <i>Saccharomyces paradoxus</i> Strains on Ige Production by Mouse Spleen Cells (2013)
  • Author(s): Kawahara T, Nakayama D, Toda K, Inagaki S, Tanaka K, Yasui H.
  • Journal Title: Food Science and Technology Research Volume: 19 Issue: 6 Pages: 1019-1027
  • DOI: 10.3136/fstr.19.1019
  • NAID: 130003392393
  • ISSN: 1344-6606, 1881-3984
  • Peer Reviewed
• [Presentation] Lactobacillus属乳酸菌抽出物塗布のアトピー性皮膚炎発症に及ぼす効果 (2016)
  • Author(s): 河原岳志、杉山 京、伊藤綾花、田口 丈
  • Organizer: 日本畜産学会
  • Place of Presentation: 日本獣医生命科学大学
  • Year and Date: 2016-03-29
• [Presentation] 乳酸菌の皮膚角化細胞における脱ユビキチン化酵素誘導によるTARC発現抑制の可能性 (2015)
  • Author(s): 原 大貴、杉山 京、河原岳志
  • Organizer: 日本酪農科学会
  • Place of Presentation: 十勝プラザ
  • Year and Date: 2015-09-25
• [Presentation] Lactobacillus属乳酸菌の皮膚角化細胞ケモカイン抑制機構の解析 (2015)
  • Author(s): 河原岳志，杉山 京
  • Organizer: 日本畜産学会 第119回大会
  • Place of Presentation: 宇都宮大学 峰キャンパス
  • Year and Date: 2015-03-29
• [Presentation] 酒造用野生酵母Saccharomyces cerevisiae S03株の NC/Nga系マウスにおけるアトピー性皮膚炎抑制作用 (2014)
  • Author(s): 河原岳志，渡辺毅哉，中山大地，保井久子
  • Organizer: 日本農芸化学会創立90周年・中部支部創立60周年記念 第171回 例会
  • Place of Presentation: 名古屋大学シンポジオン
  • Year and Date: 2014-10-11
• [Presentation] Lactobacillus属乳酸菌の皮膚角化細胞TARC発現に対する抑制効果 (2014)
  • Author(s): 河原岳志，半澤七海
  • Organizer: 日本酪農科学会 酪農科学シンポジウム
  • Place of Presentation: 昭和女子大学 グリーンホール
  • Year and Date: 2014-09-12
• [Presentation] Lactobacillus属乳酸菌の皮膚角化細胞ケモカイン発現に対する抑制効果 (2014)
  • Author(s): 河原岳志、半澤七海
  • Organizer: 日本畜産学会
  • Place of Presentation: つくば国際会議場（エポカルつくば）
• [Presentation] 朴葉熱水抽出物中に存在する ロタウイルス感染抑制成分の解析 (2014)
  • Author(s): 河原岳志、友野拓真、濵渦康範、田中勝已、保井久子
  • Organizer: 日本農芸化学会
  • Place of Presentation: 明治大学 生田キャンパス
• [Presentation] IgE抑制性野生酵母Saccharomyces paradoxus P01株のNC/Nga系マウスにおけるアトピー性皮膚炎抑制作用 (2013)
  • Author(s): 中山大地、田中勝已、保井久子、河原岳志
  • Organizer: 日本食品科学工学会
  • Place of Presentation: 実践女子大学
• [Presentation] Saccharomyces属酵母野生株によるブドウ果汁発酵物のマスト細胞脱顆粒反応に及ぼす抑制効果 (2013)
  • Author(s): 西井 慈、中山大地、田中勝已、保井久子、河原岳志
  • Organizer: 日本食品科学工学会
  • Place of Presentation: 実践女子大学
• [Patent(Industrial Property Rights)] マルメロ抽出物 (2015)
  • Inventor(s): 濱渦康範、河原岳志、井ノ上利文
  • Industrial Property Rights Holder: 株式会社ジュポンインターナショナル、国立大学法人信州大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-04-01
  • Acquisition Date: 2016-03-11