Horizontally transferred genetic elements in the tsetse fly genome

• Project/Area Number: 25850195
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: NAKAO RYO 北海道大学, (連合)獣医学研究科, 特任助教 (50633955)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 遺伝子水平伝播 / 吸血性節足動物 / ツェツェバエ / ベクター / BLSOM / Wolbachia / 共生 / ゲノム進化

Outline of Final Research Achievements

Tsetse flies (Glossina spp.) are the primary vectors of trypanosomes, which can cause Human and Animal African trypanosomiasis. The objective of this study was to explore the genetic elements horizontally transferred from microorganisms in the genome of Glossina morsitans using a composition-based bioinformatics program, so-called Batch Learning Self-Organizing Map (BLSOM). The method allows clustering of the sequence fragments based on the similarity of oligonucleotide frequencies independently of homologous sequences. After initial scan of potential HGT events by BLSOM, nearly 3.8% of the tsetse fly genome was assigned as potential HGT candidates. In order to find potential donors of HGT elements, bacterial communities in the field-captured tsetse flies were investigated. In addition to the previously recognized arthropod symbionts including genera Arsenophonus and Wolbachia, the bacteria of which genetic elements were found in tsetse genome were identified in the tsetse flies.

Research Products

• [Journal Article] Molecular survey of Babesia infections in cattle from different areas of Myanmar (2016)
  • Author(s): Bawm S, Htun LL, Maw NN, Ngwe T, Tosa Y, Kon T, Kaneko C, Nakao R, Sakurai T, Kato H, Katakura K
  • Journal Title: Ticks Tick Borne Dis. Volume: ７ Issue: 1 Pages: 204-207
  • DOI: 10.1016/j.ttbdis.2015.10.010
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular evidence of spotted fever group rickettsiae and Anaplasmataceae from ticks and stray dogs in Bangladesh (2016)
  • Author(s): Qiu Y, Nakao R, Thu MJ, Akter S, Alam MZ, Kato S, Katakura K, Sugimoto C
  • Journal Title: Parasitol Res Volume: 115 Issue: 3 Pages: 949-955
  • DOI: 10.1007/s00436-015-4819-y
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Molecular detection of Rickettsia africae in Amblyomma variegatum collected from Sudan (2015)
  • Author(s): Nakao R, Qiu Y, Salim B, Hassan SM, Sugimoto C
  • Journal Title: Vector Borne Zoonotic Dis Volume: 15 Issue: 5 Pages: 323-325
  • DOI: 10.1089/vbz.2014.1748
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Studies of trypanosomiasis in the Luangwa valley, north-eastern Zambia (2015)
  • Author(s): Laohasinnarong D, Goto Y, Asada M, Nakao R, Hayashida K, Kajino K, Kawazu S, Sugimoto C, Inoue N, Namangala B
  • Journal Title: Parasit Vectors Volume: 8 Issue: 1 Pages: 497-497
  • DOI: 10.1186/s13071-015-1112-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular characterization and specific detection of Anaplasma species (AP-sd) in sika deer and its first detection in wild brown bears and rodents in Hokkaido, Japan (2015)
  • Author(s): Moustafa MA, Lee K, Taylor K, Nakao R, Sashika M, Shimozuru M, Tsubota T
  • Journal Title: Infect Genet Evol Volume: 36 Pages: 268-274
  • DOI: 10.1016/j.meegid.2015.09.027
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Microbial population analysis of the salivary glands of ticks; a possible strategy for the surveillance of bacterial pathogens. (2014)
  • Author(s): Qiu Y, Nakao R, Ohnuma A, Kawamori F, Sugimoto C
  • Journal Title: PLoS One Volume: 9 Issue: 8 Pages: e103961-e103961
  • DOI: 10.1371/journal.pone.0103961
  • Peer Reviewed / Open Access
• [Journal Article] Detection and characterization of zoonotic pathogens of free-ranging non-human primates from Zambia (2014)
  • Author(s): Nakayima J, Hayashida K, Nakao R, Ishii A, Ogawa H, Nakamura I, Moonga L, Hang'ombe BM, Mweene AS, Thomas Y, Orba Y, Sawa H, Sugimoto C
  • Journal Title: Parasit Vectors Volume: 7 Issue: 1 Pages: 490-490
  • DOI: 10.1186/s13071-014-0490-x
  • Peer Reviewed / Open Access
• [Journal Article] Genetic diversity of Leishmania donovanl/Infantum complex in China through microsatellite analvsis. (2014)
  • Author(s): Alam, et al.
  • Journal Title: Infection Genetics and Evolution Volume: 22 Pages: 112-119
  • DOI: 10.1016/j.meegid.2014.01.019
  • Peer Reviewed
• [Journal Article] Genetic diversity among Trypanosoma (Duttonella) vivax strains from Zambia and Ghana, based on cathepsin L-like gene. (2013)
  • Author(s): Nakayima J, Nakao R, Alhassan A, Hayashida K, Namangala B, Mahama C, Afakye K, Sugimoto C
  • Journal Title: Parasite Volume: 20 Pages: 24-24
  • DOI: 10.1051/parasite/2013024
  • Peer Reviewed
• [Journal Article] First genetic detection of Coxiella burnetii in Zambian livestock. (2013)
  • Author(s): Qiu Y, Nakao R, Namangala B, Sugimoto C
  • Journal Title: American Society of Tropical Medicine and Hygiene Volume: 89 Issue: 3 Pages: 518-519
  • DOI: 10.4269/ajtmh.13-0162
  • Peer Reviewed
• [Journal Article] Characterization of a surface glycoprotein from Echinococcus multilocularis and its mucosal vaccine potential in dogs (2013)
  • Author(s): Kouguchi, H., Matsumoto, J., Nakao, R., Yamano, K., Oku, Y., and Yagi, K.
  • Journal Title: PLoS ONE Volume: 8 Issue: 7 Pages: e69821-e69821
  • DOI: 10.1371/journal.pone.0069821
  • Peer Reviewed
• [Journal Article] High prevalence of spotted fever group rickettsiae in Amblyomma variegatum from Uganda and their identification using sizes of intergenic spacers (2013)
  • Author(s): Nakao R, Qiu Y, Igarashi M, Magona JW, Zhou L, Ito K, Sugimoto C
  • Journal Title: Ticks and Tick-Borne Disease Volume: 4(6) Issue: 6 Pages: 506-512
  • DOI: 10.1016/j.ttbdis.2013.07.001
  • Peer Reviewed
• [Presentation] Horizontally transferred genetic elements in the tsetse fly genome (2015)
  • Author(s): Nakao R, Funayama S, Abe T, Sugimoto C
  • Organizer: 25th International Conference of the World Association for the Advancement of Veterinary Parasitology
  • Place of Presentation: The Arena and Convention Centre, Liverpool
  • Year and Date: 2015-08-16 – 2015-08-20
• [Presentation] Horizontally transferred genetic elements in the tsetse fly genome (2015)
  • Author(s): Nakao R, Funayama S, Abe T, Sugimoto C
  • Organizer: 25th International Conference of the World Association for the Advancement of Veterinary Parasitology
  • Place of Presentation: Liverpool Cathedral
  • Year and Date: 2015-08-16
  • Int'l Joint Research
• [Presentation] Co-evolution of vector arthropods and pathogens driven by microbiomes and horizontal gene transfers (2015)
  • Author(s): Nakao R, Sugimoto C
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 長良川国際会議場、岐阜市
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Metagenomic approaches to identify potential pathogens in ticks (2014)
  • Author(s): Nakao R, Qiu Y, Abe T, Ikemura T, Sugimoto C
  • Organizer: 日本遺伝学会第86回大会
  • Place of Presentation: 長浜バイオ大学、長浜市
  • Year and Date: 2014-09-17 – 2014-09-19
  • Invited
• [Presentation] マダニ保有ウイルス叢の網羅的解析に向けた試み (2014)
  • Author(s): 邱永晋、中尾亮、杉本千尋
  • Organizer: 第3回感染症若手フォーラム
  • Place of Presentation: 「やすらぎ伊王島」海の見えるホテル（長崎市）
• [Presentation] Tick metagenomics: a novel approach to combat emerging tick-borne diseases （ダニ媒介性新興感染症対策としてのメタゲノム解析） (2014)
  • Author(s): Nakao R, Abe T, Nijhof AM, Yamamoto S, Jongejan F, Ikemura T, Sugimoto C
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀（東京）
  • Invited
• [Presentation] マダニ媒介性フレボウイルスの検出 (2013)
  • Author(s): 中尾亮、梶原将大、松野啓太、邱永晋、森亜紀奈、海老原秀喜、高田礼人、杉本千尋
  • Organizer: 第21回ダニと疾患のインターフェースに関するセミナー
  • Place of Presentation: 稚内市少年自然の家（稚内市）
• [Presentation] 次世代シーケンス技術を活用したマダニ保有菌種組成解析 (2013)
  • Author(s): 邱永晋、中尾亮、大沼愛子、杉本千尋
  • Organizer: 第21回ダニと疾患のインターフェースに関するセミナー
  • Place of Presentation: 稚内市少年自然の家（稚内市）
• [Presentation] マダニ媒介性病原体研究における次世代シーケンサーの応用 (2013)
  • Author(s): 中尾亮、邱永晋、阿部貴志、杉本千尋
  • Organizer: NGS現場の会・第3回研究会
  • Place of Presentation: 神戸国際会議場（神戸市）
• [Presentation] Analysis on tick microbial populations using next generation sequence technique (2013)
  • Author(s): Qiu Y, Nakao R, Ohnuma A, Sugimoto C
  • Organizer: The 12th Awaji Intetrnational Forum on Infection and Immunity
  • Place of Presentation: Awaji Yumebutai International Conference Center (Awaji)
• [Presentation] エキノコックス由来表面糖タンパク質の性質とそのイヌにおける潜在的粘膜ワクチン効果 (2013)
  • Author(s): 孝口裕一、松本淳、中尾亮、山野公明、奥祐三郎、入江隆夫、八木欣平
  • Organizer: 第86回日本生化学学会大会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] Chlamydiae in ticks: Potential pathogens to humans and animals? (2013)
  • Author(s): Nakao R, Qiu Y, Sugimoto C
  • Organizer: The 1st Sapporo Summer Seminar for One Health (SaSSOH)
  • Place of Presentation: Hokkaido University (Sapporo)
• [Presentation] Analysis of tick microbial populations using next genera- tion sequence technique (2013)
  • Author(s): Qiu Y, Nakao R, Ohnuma A, Sugimoto C
  • Organizer: The 1st Sapporo Summer Seminar for One Health (SaSSOH)
  • Place of Presentation: Hokkaido University (Sapporo)
• [Presentation] 多包条虫感染イヌの血清および腸管粘液を利用した終宿主粘膜ワクチン抗原候補の探索
  • Author(s): 孝口裕一、松本淳、中尾亮、山野公明、奥祐三郎、入江隆夫、八木欣平
  • Organizer: 第59回日本寄生虫学会・日本衛生動物学会 北日本支部合同大会
  • Place of Presentation: 酪農学園大学（江別市）