Identification and structual analysis of cell adhesion molecules of Trypanosoma congolense

• Project/Area Number: 25850196
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Jikei University School of Medicine
• Principal Investigator: TATSUYA Sakurai 東京慈恵会医科大学, 医学部, 講師 (60547777)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 原虫 / 細胞表面タンパク質 / 細胞接着 / 細胞表面蛋白質 / 質量分析 / 細胞表面分子 / 感染症 / ベクター / 宿主

Outline of Final Research Achievements

This study aimed at identification of cell adhesion molecules of Trypanosoma congolense epimastigotes and bloodstream forms, and isolation of candidate molecules with biochemical and molecular methods followed by its identification with mass spectrometry analyses were carried out. As the result of that, several surface proteins of unknown function and one unknown protein interacting with sialic acid were yielded as candidate proteins from epimastigotes and bloodstream forms, respectively.

Research Products

• [Journal Article] Case report: protein-losing enteropathy caused by Mesocestoides vogae (syn. M. corti) in a dog. (2014)
  • Author(s): Tamura Y, Ohta H, Kashiide T, Matsumoto J, Sakurai T, Yokoyama N, Morishita K, Nakamura K, Yamasaki M, Takiguchi M.
  • Journal Title: Veterinary Parasitology Volume: 205 Issue: 1-2 Pages: 412-5
  • DOI: 10.1016/j.vetpar.2014.07.027
  • Peer Reviewed
• [Journal Article] Molecular prevalence and genetic diversity of bovine Theileria orientalis in Myanmar (2014)
  • Author(s): Bawm S, Shimizu K, Hirota J, Tosa Y, Htun LL, Maw NN, Thein M, Kato H, Sakurai T, Katakura K
  • Journal Title: Parasitol Int Volume: (in press) Issue: 4 Pages: 640-645
  • DOI: 10.1016/j.parint.2014.04.009
  • Peer Reviewed
• [Journal Article] Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection (2014)
  • Author(s): Ozelu, C.O., Gomez, E.A., Caceres, A.G., Sakurai, T., Martini, R.L., Uezato, H., Mimori, T., Katakura, K., Hashiguchi, Y., Kato, H.
  • Journal Title: Acta Tropica Volume: 132 Pages: 1-6
  • DOI: 10.1016/j.actatropica.2013.12.016
  • NAID: 120005367442
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] First detection of Leishmania tropica DNA and Trypanosoma species in Sergentomyia sand flies (Diptera: Psychodidae) from an outbreak area of cutaneous leishmaniasis in Ghana. (2014)
  • Author(s): Nzelu CO, Kato H, Puplampu N, Desewu K, Odoom S, Wilson MD, Sakurai T, Katakura K, Boakye DA.
  • Journal Title: PLoS Negl Trop Dis. Volume: 8 Issue: 2 Pages: e2630-e2630
  • DOI: 10.1371/journal.pntd.0002630
  • Peer Reviewed
• [Journal Article] Genetic diversity of Leishmania donovanl/Infantum complex in China through microsatellite analvsis. (2014)
  • Author(s): Alam, et al.
  • Journal Title: Infection Genetics and Evolution Volume: 22 Pages: 112-119
  • DOI: 10.1016/j.meegid.2014.01.019
  • Peer Reviewed
• [Journal Article] Genetic diversity of the mitochondrial cytochrome b gene in Lutzomyia spp., with special reference to Lutzomyia peruensis, a main vector of Leishmania (Viannia) peruviana in the Peruvian Andes (2013)
  • Author(s): Yamamoto K, Caceres AG, Gomez EA, Mimori T, Iwata H, Korenaga M, Sakurai T, Katakura K, Hashiguchi Y, Kato H
  • Journal Title: Acta Trop Volume: 126 Issue: 2 Pages: 156-163
  • DOI: 10.1016/j.actatropica.2013.02.007
  • NAID: 120005228382
  • Peer Reviewed