| Project/Area Number |
25850199
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Nagasaki University |
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Principal Investigator |
ASADA Masahito 長崎大学, 熱帯医学研究所, 助教 (40587028)
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| Research Collaborator |
YAHATA Kazuhide 長崎大学, 熱帯医学研究所, 助教
HAKIMI Hassan 長崎大学, 熱帯医学研究所, 特任研究員
YOKOYAMA Naoaki 帯広畜産大学, 原虫病研究センター, 教授
IGARASHI Ikuo 帯広畜産大学, 原虫病研究センター, 教授
KANEKO Osamu 長崎大学, 熱帯医学研究所, 教授
SUAREZ E. Carlos ワシントン州立大学, 獣医学部, 准教授
KAWAZU Shin-ichiro 帯広畜産大学, 原虫病研究センター, 教授
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | バベシア / ウシ / 赤血球侵入 / 滑走運動 / 遺伝子組換え / イメージング / バベシア原虫 / 遺伝子改変 / 獣医寄生虫 / 原虫病学 / 獣医寄生虫学 / バイオイメージング |
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| Outline of Final Research Achievements |
Babesia bovis is an intraerythrocytic apicomplexan parasite, which is transmitted by ticks and causes the disease bovine babesiosis in cattle. In this study, we developed transfection system on B. bovis by using double selection with WR99210 and blasticidin-S, which enables us to perform double transfection in the parasite. Then, we analyzed gliding motility of B. bovis merozoite by disrupting TRAP gene families. Moreover, we analyzed the egress mechanism of the parasite. We found egress was artificially induced in vitro using calcium ionophore A23187 and thapsigargin which increase Ca(2+) concentration in the cytosol of the parasite cells. These finding will contribute to develop new therapeutic strategies to bovine babesiosis.
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