Idntification of novel tumor antigens of canine B-cell lymphoma and development of diagnostic and therapeutic application.

• Project/Area Number: 25850211
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Osaka Prefecture University
• Principal Investigator: Furuya Masaru 大阪府立大学, 生命環境科学研究科(系), 助教 (30500706)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 腫瘍抗原 / 犬 / リンパ腫

Outline of Final Research Achievements

Tumor-associated antigens (TAAs), which are produced in tumor cells, are applied as biological markers, tumor vaccine antigens, and molecular targets of therapeutic drugs. In this study, we aimed to identify the TAAs of canine B cell lymphoma to develop novel diagnostic and therapeutic procedures by serological immunoscreening with canine testicular cDNA expression library.
In this study, we have primarily identified 26 different TAAs by immunoscreening. The results of serological mini-arrays of identified antigens showed that 13 of 17 sera of lymphoma-dog reacted with some antigens in contrast to 10 healthy dogs reacted with no antigens. These findings will contribute to the development of diagnostic technologies for patient dogs, although it is needed that establishment of quantitative ELISA system to measure serum antibody titer to those antigens.

Research Products

• [Journal Article] A Serological Survey of Leptospira Spp. Antibodies in Wild Raccoons (Procyon lotor) in Osaka, Japan. (2016)
  • Author(s): Saeki J, Nakanishi H, Masubuchi K, Matsubayashi M, Furuya M, Tani H and Sasai K.
  • Journal Title: Asian Journal of Animal and Veterinary Advances Volume: 11 Issue: 4 Pages: 258-262
  • DOI: 10.3923/ajava.2016.258.262
  • Peer Reviewed
• [Journal Article] Localization of heat shock protein 110 in canine mammary gland tumor (2015)
  • Author(s): Satoru Okada, Masaru Furuya, Shigeo Takenaka, Ayano Fukui, Makoto Matsubayashi, Hiroyuki Tani, Kazumi Sasai
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 167(3-4) Issue: 3-4 Pages: 139-46
  • DOI: 10.1016/j.vetimm.2015.07.012
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characteristics of bone fractures and usefulness of micro-computed tomography for fracture detection in rabbits: 210 cases (2007-2013) (2015)
  • Author(s): Sasai H, Fujita D, Tagami Y, Seto E, Denda Y, Hamakita H, Ichihashi T, Okamura K, Furuya M, Tani H, Sasai K, Yamate J.
  • Journal Title: Journal of the American Veterinary Medical Association Volume: 246 Issue: 12 Pages: 1339-1345
  • DOI: 10.2460/javma.246.12.1339
  • Peer Reviewed
• [Journal Article] The detection of toxigenic Corynebacterium ulcerans from cats with nasal inflammation in Japan. (2015)
  • Author(s): Saeki J, Katsukawa C, Matsubayashi M, Nakanishi H, Furuya M, Tani H, Sasai K.
  • Journal Title: Epidemiol Infect. Volume: 1 Issue: 12 Pages: 1-6
  • DOI: 10.1017/s0950268814003550
  • Peer Reviewed / Open Access
• [Journal Article] Characteristics of bone fractures and usefulness of micro–computed tomography for fracture detection in rabbits: 210 cases (2007–2013) (2015)
  • Author(s): Sasai H, Fujita D, Tagami Y, Seto E, Denda Y, Hamakita H, Ichihashi T, Okamura K, Furuya M, Tani H, Sasai K, Yamate J.
  • Journal Title: J Am Vet Med Assoc. Volume: 246
  • Peer Reviewed
• [Journal Article] Profiling of Serum Metabolites in Canine Lymphoma Using Gas Chromatography Mass Spectrometry (2014)
  • Author(s): Tamai R, Furuya M, Hatoya S, Akiyoshi H, Yamamoto R, Komori Y, Yokoi S, Tani K, Hirano Y, Komori M, Takenaka S.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 11 Pages: 1513-1518
  • DOI: 10.1292/jvms.14-0043
  • NAID: 130004677727
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Identification of novel tumor-associated antigens in canine mammary gland tumor. (2013)
  • Author(s): Furuya M., Funasaki M., Tani H., Sasai K.
  • Journal Title: Veterinary and Comparative Oncology. Volume: 10.1111 Issue: 3 Pages: 194-202
  • DOI: 10.1111/vco.12034
  • Peer Reviewed
• [Presentation] Identification of novel tumor-associated antigens in canine mammary gland tumor (2015)
  • Author(s): Furuya M, Funasaki M, Okada S, Tani H & Sasai K
  • Organizer: Asian Meeting of Animal Medicine Specialties 2015
  • Place of Presentation: マレーシア, クアラルンプール, ルネッサンスホテル
  • Year and Date: 2015-11-01
  • Int'l Joint Research
• [Presentation] 犬のリンパ腫における血液代謝物プロファイル解析 (2015)
  • Author(s): 田舞理央、石塚泰雄、佐伯潤、横井愼一、松岡聰、古家優、鳩谷晋吾、秋吉秀保、竹中重雄、小森雅之
  • Organizer: 第11回 日本獣医内科学アカデミー学術大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2015-02-20 – 2015-02-22
• [Presentation] イヌの乳腺腫瘍における新規腫瘍抗原の同定 (2014)
  • Author(s): 古家優、船崎正治、谷浩行、笹井和美
  • Organizer: 第157回 日本獣医学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] イヌのT細胞型リンパ腫における新規腫瘍抗原の同定 (2014)
  • Author(s): 川口太平、古家優、谷浩行、笹井和美
  • Organizer: 第157回 日本獣医学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] 鶏サルモネラ症防除を目指した大腸菌を用いた組換え不活化経口ワクチンの開発 (2014)
  • Author(s): 稲垣果歩、谷浩行、 小西翔、松林誠、 古家優、笹井和美
  • Organizer: 第157回 日本獣医学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12