| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The purpose of this study is to investigate the possibility of stem cell and olfactory ensheathing cell transplantation to naturally occurred spinal cord injury in dogs.To establish the methods, it was analized whether stem cells and olfactory ensheathing could be harvested from canine nasal mucosa of which were obtained via nasal cavity using endoscopy. Some cells expressed stem cell markers on nasal mucosa section, and other cells expressed olfactory ensheathing cell marker. In addtion, those cells could be cultured. However infection were seen in few cases. Therefore further methods would be needed for this study from safety.
To find altanative cell source, de-defferentiated fat cells (DFAT),which are guaranteed the safety,were transplanted to spinal cord injury model mice to analyze the efficacy and safety. DFAT transplanted mice showed significantly better walking steps than control mice.
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