Development of treatment technology for bovine mastitis by recombinant bovine cytokine intramammary infusion at dry-off.
Project/Area Number |
25850215
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Veterinary medical science
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Research Institution | National Agriculture and Food Research Organization |
Principal Investigator |
KIKU Yoshio 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門病態研究領域, 上級研究員 (70370179)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 乳房炎 / 牛 / サイトカイン / 治療 / 予防 / 乳用牛 / GM-CSF / 治療・予防 / 乾乳期 / 遺伝子組換えカイコ |
Outline of Final Research Achievements |
The objective of this study was to investigate the effect for treatment or prevention of bovine mastitis of the intramammary infusion of recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) produced in transgenic silkworm as a dry cow therapy. As the treatment or preventive trials, the infected or non-infected quarter were assigned at drying-off to an intramammary infusion of rbGM-CSF (400 μg/quarter) solutions. Each milk sample was collected for bacteriological examination and somatic cell count (SCC) at dry-off and a month after calving. In the treatment trial, total bacterial counts and SCC in milk was lower at 0, 7, 14 and 28 days after calving than at dry-off. In the prevention trial, the new intramammary infection of all cows was not detected after calving. These results suggest that the intramammary infusion of rbGM-CSF at drying off has a high potential as an agent of dry cow therapy.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Extracellular cyclophilin A possesses chemotaxic activity in cattle2015
Author(s)
Takanashi S, Nochi T, Abe M, Itaya N, Urakawa M, Sato K, Zhuang T, Umemura S, Hayashi T, Kiku Y, Kitazawa H, Rose MT, Watanabe K, Aso H
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Journal Title
Vet Res
Volume: 46
Issue: 1
Pages: 80-89
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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