Molecular mechanism underlying juvenile hormone-mediated repression of insect pupation

• Project/Area Number: 25850230
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Insect science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: Kayukawa Takumi 国立研究開発法人農業生物資源研究所, 昆虫科学研究領域・昆虫成長制御研究ユニット, 主任研究員 (70580463)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: juvenile hormone / ecdysone / Kruppel homolog 1 / Broad complex / metamorphosis / cis-element / trans-element / Bombyx mori / broad complex / bombyx mori / 幼若ホルモン / エクダイソン / 脱皮 / 変態 / カイコ

Outline of Final Research Achievements

Juvenile hormone (JH) is known to repress pupal metamorphosis in insects. However, the molecular mechanism underlying the JH-mediated repression of pupation has remained unclear. In this study, I focused on a pupal specifier gene and analyzed the molecular mechanism. As a result, JH-inducible protein (Kruppel homolog 1) directly represses transcription of pupal specifier gene and thereby represses insect pupation.

Research Products

• [Int'l Joint Research] CSIC-UPF(Spain)
• [Int'l Joint Research] ASCR/University of South Bohemia(チェコ)
• [Int'l Joint Research] CSIRO(Australia)
• [Journal Article] Krüppel homolog 1 inhibits insect metamorphosis via direct transcriptional repression of Broad-complex, a pupal specifier gene (2016)
  • Author(s): Kayukawa T, Nagamine K, Ito Y, Nishita Y, Ishikawa Y, Shinoda T
  • Journal Title: The Journal of Biological Chemistry Volume: 291 Issue: 4 Pages: 1751-1751
  • DOI: 10.1074/jbc.m115.686121
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Juvenile hormone related genes as targets for developing biorational insecticides (2015)
  • Author(s): 篠田徹郎、大門高明、古田賢次郎、粥川琢己
  • Journal Title: Japanese Journal of Pesticide Science Volume: 40 Issue: 1 Pages: 58-67
  • DOI: 10.1584/jpestics.W14-37
  • NAID: 130005096500
  • ISSN: 2187-0365, 2187-8692
• [Journal Article] Functional characterization of two paralogous JH receptors, methoprene-tolerant 1 and 2, in the silkworm, Bombyx mori (2015)
  • Author(s): Kayukawa T, Shinoda T
  • Journal Title: Applied Entomology and Zoology Volume: 50 Pages: 383-383
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Functional characterization of two paralogous JH receptors, methoprene-tolerant 1 and 2, in the silkworm, Bombyx mori (Lepidoptera: Bombycidae) (2015)
  • Author(s): Takumi Kayukawa, Tetsuro Shinoda
  • Journal Title: Applied Entomology and Zoology Volume: 50 Issue: 3 Pages: 383-391
  • DOI: 10.1007/s13355-015-0345-8
  • NAID: 40020571183
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A role for Taiman in insect metamorphosis (2014)
  • Author(s): Lozano J, Kayukawa T, Shinoda T, Belles X
  • Journal Title: PLoS Genetics Volume: 10 Issue: 10 Pages: e1004769-e1004769
  • DOI: 10.1371/journal.pgen.1004769
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Hormonal regulation and developmental role of Kruppel homolog 1, a repressor of metamorphosis, in the silkworm Bombyx mori (2014)
  • Author(s): Kayukawa T, Murata M, Kobayashi I, Muramatsu D, Okada C, Uchino K, Sezutsu H, Kiuchi M, Tamura T, Hiruma K, Ishikawa Y, Shinoda T
  • Journal Title: Developmental Biology Volume: 388 Issue: 1 Pages: 48-56
  • DOI: 10.1016/j.ydbio.2014.01.022
  • Peer Reviewed
• [Presentation] How does juvenile hormone prevent pupal metamorphosis in holometabolous insects ? (2016)
  • Author(s): Kayukawa T, Shinoda T
  • Organizer: International Congress of Entomology
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 2016-09-25
  • Int'l Joint Research
• [Presentation] 幼若ホルモンによる成虫化決定因子E93の抑制分子機構 (2016)
  • Author(s): 粥川琢巳, 篠田徹郎, 伊藤由果
  • Organizer: 日本応用動物昆虫学会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2016-03-26
• [Presentation] 幼若ホルモンによるエクダイソン誘導性蛹化決定因子Broad-complexの抑制分子機構 (2015)
  • Author(s): 粥川琢巳, 長峯啓佑, 石川幸男, 西田義憲, 篠田徹郎
  • Organizer: 第59回日本応用動物昆虫学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] カイコの２種Methoprene tolerantパラログの機能解析 (2015)
  • Author(s): 粥川琢巳, 篠田徹郎
  • Organizer: 第59回日本応用動物昆虫学会大会
  • Place of Presentation: 山形大学
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Juvenile hormone signalling analysis using T. castaneum cell line (2014)
  • Author(s): Kayukawa T, Tateishi K, Shinoda T
  • Organizer: 10th International Conference on Juvenile Hormones
  • Place of Presentation: つくば
  • Year and Date: 2014-06-09 – 2014-06-13
• [Presentation] JH signalling molecules as targets for developing new IGRs (2014)
  • Author(s): Shinoda T, Kayukawa T, Furuta K, Daimon T
  • Organizer: 10th International Conference on Juvenile Hormones
  • Place of Presentation: つくば
  • Year and Date: 2014-06-09 – 2014-06-13
• [Presentation] Importance of JH signaling arises with competence of larvae to metamorphose (2014)
  • Author(s): Smykal V, Daimon T, Kayukawa T, Takaki K, Shinoda T, Jindra M
  • Organizer: 10th International Conference on Juvenile Hormones
  • Place of Presentation: つくば
  • Year and Date: 2014-06-09 – 2014-06-13
• [Presentation] HTSスクリーニングに向けたJHスクリーニング系の開発 (2014)
  • Author(s): 粥川琢巳, 古田賢次郎, 篠田徹郎
  • Organizer: 第58回日本応用動物昆虫学会大会
  • Place of Presentation: 高知大学
• [Remarks] プレスリリース： 幼若ホルモンがサナギ化を抑えるメカニズムを解明
  • URL: http://www.nias.affrc.go.jp/press/2015/20151201/