Analysis on a novel plant immune system triggered by a virus-encoded molecular pattern.

• Project/Area Number: 25850243
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: The University of Tokyo
• Principal Investigator: HASHIMOTO Masayoshi 東京大学, 農学生命科学研究科, 特任助教 (20615273)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 普遍的分子パターン / 植物ウイルス / 細胞死 / 両親媒性ヘリックス

Outline of Final Research Achievements

In this study, we aimed to reveal a novel plant immune system induced by a virus-encoded molecular pattern. As a result, we found an elicitor of immune responses in the N-terminal region of helicase proteins encoded by a comovirus. In this region, there is a predicted amphipathic helix involved in virus replication. We showed that the amphipathic helix is required for the induction of immune responses. In addition, we revealed that the elicitor activity is tightly correlated with the membrane modification activity, which is essential for the role of the amphipathic helix in virus replication.

Research Products

• [Journal Article] Cell death triggered by a putative amphipathic helix of radish mosaic virus helicase protein is tightly correlated with host membrane modification. (2015)
  • Author(s): Hashimoto M., Komatsu K., Iwai R., Keima T., Maejima K., Shiraishi T., Ishikawa K., Yoshida T., Kitazawa Y., Okano Y., Yamaji Y., Namba S.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 28 Issue: 6 Pages: 675-88
  • DOI: 10.1094/mpmi-01-15-0004-r
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Complete nucleotide sequence and genome structure of a Japanese isolate of hibiscus latent Fort Pierce virus, a unique tobamovirus that contains an internal poly(A) region in its 3' end (2014)
  • Author(s): Yoshida T., Kitazawa Y., Komatsu K., Neriya Y., Ishikawa K., Fujita N., Hashimoto M., Maejima K., Yamaji Y., Namba S
  • Journal Title: Archives of Virology Volume: 159 Issue: 11 Pages: 3161-3165
  • DOI: 10.1007/s00705-014-2175-3
  • Peer Reviewed
• [Journal Article] In Planta Recognition of a Double-Stranded RNA Synthesis Protein Complex by a Potexviral RNA Silencing Suppressor. (2014)
  • Author(s): Okano Y., Senshu H., Hashimoto M., Neriya Y., Netsu O., Minato N., Yoshida T., Maejima K., Oshima K., Komatsu K., Yamaji Y., Namba S.
  • Journal Title: Plant Cell Volume: 26 Issue: 5 Pages: 2168-2183
  • DOI: 10.1105/tpc.113.120535
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development of an on-site plum pox virus detection kit based on immunochromatography. (2014)
  • Author(s): Maejima K., Himeno M., Netsu O., Ishikawa K., Yoshida T., Fujita N., Hashimoto M., Komatsu K., Yamaji Y., Namba S.
  • Journal Title: Journal of General Plant Pathology Volume: 80 Issue: 2 Pages: 176-183
  • DOI: 10.1007/s10327-014-0504-8
  • Peer Reviewed
• [Journal Article] Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement. (2013)
  • Author(s): 石川 一也
  • Journal Title: Journal of General Virology Volume: 94 Issue: 3 Pages: 682-686
  • DOI: 10.1099/vir.0.047860-0
  • Peer Reviewed
• [Journal Article] First report of tomato chlorotic dwarf viroid isolated from symptomless petunia plants (Petunia spp.) in Japan. (2013)
  • Author(s): 白石 拓也
  • Journal Title: Journal of General Plnat Pathology Volume: (印刷中) Issue: 3 Pages: 214-216
  • DOI: 10.1007/s10327-013-0444-8
  • Peer Reviewed
• [Journal Article] Construction of an infectious cDNA clone of radish mosaic virus, a crucifer-infecting comovirus. (2013)
  • Author(s): 小松 健
  • Journal Title: Archives of Virology Volume: (印刷中) Issue: 7 Pages: 1579-1582
  • DOI: 10.1007/s00705-013-1635-5
  • Peer Reviewed
• [Presentation] ダイコンモザイクウイルスの感染による茎頂壊死の誘導因子 (2015)
  • Author(s): 橋本将典・遊佐礼・友光達哉・桂馬拓也・岡野夕香里・小松健・山次康幸・難波成任
  • Organizer: 日本植物病理学会創立100周年記念大会
  • Place of Presentation: 明治大学駿河台キャンパス
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] plantago asiatica mosaic virusのTGBp1は植物内在性の小分子RNA「tasiRNA」の生成経路を抑制する (2015)
  • Author(s): 二條貴通・岡野夕香里・煉谷裕太朗・橋本将典・遊佐礼・桂馬拓也・大島研郎・小松健・山次康幸・難波成任
  • Organizer: 日本植物病理学会創立100周年記念大会
  • Place of Presentation: 明治大学駿河台キャンパス
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] A linear signaling pathway formed by three MAPKKKs leading to systemic necrosis induced by a potexvirus in Nicotiana benthamiana (2013)
  • Author(s): Minato Nami, Masayoshi Hashimoto, Kensaku Maejima, Shigetou Namba
  • Organizer: EMBO Workshop Green viruses, from gene to landscape
  • Place of Presentation: Hyeres-les-Plamiers, France
• [Book] 植物ウイルス病研究会レポート (2013)
  • Author(s): 小松健・橋本将典・山次康幸・難波成任
  • Total Pages: 91
  • Publisher: 植物ウイルス病研究会