| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
To elucidate the recycling mechanism and function of vacuolar carbohydrate in yeast, I focused on regulatory mechanism of Ams1 enzyme respond to nutrition.
As a result, it was found that this enzyme was strongly upregulated respond to starvation at both transcription level and post-translationl level. It was also revealed that this enzyme was tightly suppressed by TORC1 in nutrient rich condition, but induced by a stress-induced transcritional factor and by protease dependent processing in the vacuoles. These results suggested that yeast may recycle degradation product of intracellular mannnose in the vacuole especially in starved condition.
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