Analyses of recycling mechanism and function of vacuolar carbohydrate in yeast

• Project/Area Number: 25850246
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Ritsumeikan University
• Principal Investigator: Umekawa Midori 立命館大学, 生命科学部, 助教 (60633097)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: nutrition sensing / carbohydrate metabolism / 栄養応答 / 糖代謝 / ストレス応答 / 栄養飢餓 / 糖質分解 / 出芽酵母

Outline of Final Research Achievements

To elucidate the recycling mechanism and function of vacuolar carbohydrate in yeast, I focused on regulatory mechanism of Ams1 enzyme respond to nutrition.
As a result, it was found that this enzyme was strongly upregulated respond to starvation at both transcription level and post-translationl level. It was also revealed that this enzyme was tightly suppressed by TORC1 in nutrient rich condition, but induced by a stress-induced transcritional factor and by protease dependent processing in the vacuoles. These results suggested that yeast may recycle degradation product of intracellular mannnose in the vacuole especially in starved condition.

Research Products

• [Journal Article] The signaling pathways underlying starvation-induced upregulation of α-mannosidase Ams1 in Saccharomyces cerevisiae (2016)
  • Author(s): Umekawa M, Ujihara M, Makishima K, Yamamoto S, Takematsu H, Wakayama M.
  • Journal Title: Biochim Biophys Acta. Volume: 1860 Issue: 6 Pages: 1192-1201
  • DOI: 10.1016/j.bbagen.2016.02.018
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (2016)
  • Author(s): Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, Abeliovich H, Acevedo Arozena A, Adachi H, Adams CM, Adams PD, Adeli K, Adhihetty PJ, Adler SG, Agam G, Agarwal R, Aghi MK, Agnello M, Agostinis P, Aguilar PV, Aguirre-Ghiso J,（他省略）, Umekawa M,（他省略） (全2467名、研究代表者2144番目)
  • Journal Title: Autophagy Volume: 12 Pages: 1-222
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Novel properties of γ-glutamyltransferase from Pseudomonas syringae with β-aspartyltransferase activity (2015)
  • Author(s): Prihanto AA, Nonomura Y, Takagi K, Naohara R, Umekawa M, Wakayama M.
  • Journal Title: Biotechnol Lett. Volume: 37 Issue: 11 Pages: 2255-2263
  • DOI: 10.1007/s10529-015-1906-1
  • Peer Reviewed / Open Access
• [Journal Article] Effective treatment for suppression of acrylamide formation in fried potato chips using L-asparaginase from Bacillus subtilis (2015)
  • Author(s): Yohei Onishi, Asep A. Prihanto, Shigekazu Yano, Kazuyoshi Takagi, Midori Umekawa and Mamoru Wakayama
  • Journal Title: 3 Biotech Volume: 印刷中 Issue: 5 Pages: 783-789
  • DOI: 10.1007/s13205-015-0278-5
  • Peer Reviewed / Open Access
• [Presentation] 出芽酵母の栄養応答に関わる新規細胞表層タンパク質の機能解析 (2017)
  • Author(s): 梅川碧里、氏原将人、中井大貴、若山守
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学（京都府京都市）
  • Year and Date: 2017-03-19
• [Presentation] 出芽酵母は栄養飢餓時にalpha-マンノシダーゼAms1を誘導する (2016)
  • Author(s): 梅川碧里、氏原将人、槙島一樹、竹松弘、若山守
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-09-26
• [Presentation] Nutrient starvation induces alpha-mannosidase Ams1 in Saccharomyces cerevisiae (2016)
  • Author(s): Umekawa M, Ujihara M, Makishima K, Yamamoto S, Takematsu H, Wakayama M.
  • Organizer: The Allied Genetics Conference 2016
  • Place of Presentation: オーランド（アメリカ合衆国）
  • Year and Date: 2016-07-15
  • Int'l Joint Research
• [Presentation] 出芽酵母のα-マンノシダーゼAms1の発現制御機構の解析 (2015)
  • Author(s): 氏原将人、槙島一樹、山本祥平、若山守、梅川碧里
  • Organizer: 第88回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド（兵庫、神戸）
  • Year and Date: 2015-12-02
• [Presentation] 出芽酵母α-マンノシダーゼAMS1の活性制御機構の解析 (2015)
  • Author(s): 梅川碧里、氏原将人、槙島一樹、山本祥平、若山守
  • Organizer: 酵母遺伝学フォーラム
  • Place of Presentation: 広島大学（広島、東広島）
  • Year and Date: 2015-09-02
• [Presentation] 出芽酵母α-マンノシダーゼAMS1の活性制御機構の解析 (2015)
  • Author(s): 梅川碧里、槙島一樹、氏原将人、山本祥平、若山守
  • Organizer: 日本生化学会近畿支部例会
  • Place of Presentation: 立命館大学びわこくさつキャンパス（滋賀、草津）
  • Year and Date: 2015-05-16
• [Presentation] 出芽酵母α-マンノシダーゼAMS1の活性制御機構の解析 (2015)
  • Author(s): 中井大貴、槙島一樹、氏原将人、山本祥平、若山守、梅川碧里
  • Organizer: 日本農芸化学会
  • Place of Presentation: 岡山大学（岡山県）
  • Year and Date: 2015-03-28
• [Presentation] 出芽酵母のオートファジー制御に関わるKsp1 キナーゼの解析
  • Author(s): 梅川 碧里
  • Organizer: 酵母研究会第７７回講演会
  • Place of Presentation: 京都テルサ（京都府）
  • Invited
• [Book] 糖鎖の新機能開発・応用ハンドブック (2015)
  • Author(s): 梅川碧里、芦田久、山本憲二、他(分担執筆)
  • Total Pages: 678
  • Publisher: NTS