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Translational regulatory mechanism in during cold stress response in plants

Research Project

Project/Area Number 25850247
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Nakaminami Kentaro  国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (40513403)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsシロイヌナズナ / 低温馴化・脱馴化 / RNA制御 / 翻訳制御
Outline of Final Research Achievements

Overwintering plants exhibits high levels of cold tolerance, which is acquired through the process of cold acclimation (CA). This acquired cold tolerance is rapidly reduced during cold de-acclimation (DA) and plants resume growth after sensing warm temperatures. In order to better understand plant growth and development, it is important to decipher the functional mechanisms of the CA and DA process.
In this study, it is demonstrated that many cold-inducible genes were transcribed and translated, however a similar number of cold-inducible genes were not translated during the CA and were translated at a later stage, DA. Due to the disjunction between the up-regulation of specific genes and the lack of corresponding protein accumulation during CA, is likely that the translation of proteins during DA was primed with stored mRNA. This data imply that these are likely regulated by translational regulation, which alters protein expression patterns in response to environmental alterations.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Loss of Arabidopsis 5’-3’ exoribonuclease AtXRN4 function enhances heat stress tolerance of plants subjected to severe heat stress2015

    • Author(s)
      Anh Hai Nguyen, A. H., Matsui, A., Tanaka, M., Mizunashi, K., Nakaminami, K., Hayashi, M., Iida, K., Toyoda, T., Nguyen, D. V. and Seki, M.
    • Journal Title

      Plant Cell Physiol.

      Volume: 56 Issue: 9 Pages: 1762-1772

    • DOI

      10.1093/pcp/pcv096

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of differential expression patterns of mRNA and protein during cold- and de-acclimation in Arabidopsis.2014

    • Author(s)
      Nakaminami K, Matsui A, Nakagami H, Minami A, Nomura Y, Tanaka M, Morosawa T, Ishida J, Takahashi S, Uemura M, Shirasu K, Seki M.
    • Journal Title

      Molecular and Cellular Proteomics

      Volume: 13 Issue: 12 Pages: 3602-3611

    • DOI

      10.1074/mcp.m114.039081

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arabidopsis non-coding RNA regulation in abiotic stress responses (Review)2013

    • Author(s)
      Matsui, A. , Nguyen, A.H. , Nakaminami, K. , Seki, M.
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 14 Issue: 11 Pages: 22642-22654

    • DOI

      10.3390/ijms141122642

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Characterization of functional small peptides related to salinity stress tolerance2016

    • Author(s)
      Kentaro Nakaminami, Chihiro Ohashi, Maho Tanaka, Kousuke Hanada and Motoaki Seki
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手県,岩手大学
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Characterizing the Role of Heat Stress-Inducible Small Open Reading Frames (sORFs)2015

    • Author(s)
      BASHIR Khurram, NAKAMINAMI Kentaro, HIGUCHI Mieko, YOSHIZUMI Takeshi, OKAMOTO Masanori, TANAKA Maho, MATSUI Minami, SHINOZAKI Kazuo, HANADA Kousuke, SEKI Motoaki
    • Organizer
      26th International Conference on Arabidopsis Research
    • Place of Presentation
      Paris, France
    • Year and Date
      2015-07-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 環境ストレス応答に機能するペプチドホルモンの発見を目指して2014

    • Author(s)
      中南 健太郎
    • Organizer
      植物グローバル教育プロジェクト 平成26年度シンポジウム
    • Place of Presentation
      奈良、日本
    • Year and Date
      2014-11-18
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] RNA masking system during cold deacclimation of Arabidopsis plants2014

    • Author(s)
      Kentaro Nakaminami, Akihiro Matsui, Hirofumi Nakagami, Anzu Minami, Yuko Nomura, Maho Tanaka, Taeko Morosawa, Junko Ishida, Satoshi Takahashi, Matsuo Uemura, Ken Shirasu, and Motoaki Seki
    • Organizer
      Plant Biology 2014, American Society of Plant Biologists
    • Place of Presentation
      Portland, Oregon, USA
    • Year and Date
      2014-07-12 – 2014-07-16
    • Related Report
      2014 Research-status Report
  • [Presentation] Characterization of functional small peptides in plant abiotic stress responses2014

    • Author(s)
      Kentaro Nakaminami, Mieko Higuchi, Takeshi Yoshizumi, Masanori Okamoto, Khurram Bashir, Minami Shimizu, Chihiro Ohashi, Maho Tanaka, Minami Matsui, Kazuo Shinozaki, Motoaki Seki and Kousuke Hanada
    • Organizer
      日本植物生理学会
    • Place of Presentation
      富山県,富山大学
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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