| Project/Area Number |
25860021
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
SAIGUSA Daisuke 東北大学, 東北メディカル・メガバンク機構, 助教 (90545237)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | リゾリン脂質 / スフィンゴ脂質 / LC/MS/MS / レーザーマイクロダイセクション / ヒートスタビライザー / 微量組織切片 / 原子間力顕微鏡 / S1Pトランスポーター / 固相抽出カラム / ジルコニア / メタボローム解析 |
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| Outline of Final Research Achievements |
In this study, we developed the analytical protocol for demonstrating the intracellular distribution and gradation and localization of lysophospholipids (LPL) using a LC/MS/MS. First, we developed an ultra-high sensitive method for LPL by LC/MS/MS coupled with a column switching. Second, we have evaluated the LPL decomposition in mouse liver by a heat stabilizer. Finally, we detected and determined LPL form the frozen sections of tissue (10 μm) of mice that was cut by laser micro dissection and determined LPL by LC/MS/MS.
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