Development of determination method for lysophospholipids by LC/MS/MS
| Project/Area Number |
25860021
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
SAIGUSA Daisuke 東北大学, 東北メディカル・メガバンク機構, 助教 (90545237)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | リゾリン脂質 / スフィンゴ脂質 / LC/MS/MS / レーザーマイクロダイセクション / ヒートスタビライザー / 微量組織切片 / 原子間力顕微鏡 / S1Pトランスポーター / 固相抽出カラム / ジルコニア / メタボローム解析 |
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| Outline of Final Research Achievements |
In this study, we developed the analytical protocol for demonstrating the intracellular distribution and gradation and localization of lysophospholipids (LPL) using a LC/MS/MS. First, we developed an ultra-high sensitive method for LPL by LC/MS/MS coupled with a column switching. Second, we have evaluated the LPL decomposition in mouse liver by a heat stabilizer. Finally, we detected and determined LPL form the frozen sections of tissue (10 μm) of mice that was cut by laser micro dissection and determined LPL by LC/MS/MS.
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Report
(3 results)
Research Products
(20 results)
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[Journal Article] Overexpression of autotaxin, a lysophosphatidic acid-producing enzyme, enhances cardia bifida induced by hypo-sphingosine-1-phosphate signaling in zebrafish embryo2014
Author(s)
Nakanaga K, Hama K, Kano K, Sato T, Yukiura H, Inoue A, Saigusa D, Tokuyama H, Tomioka Y, Nishina H, Kawahara A, Aoki J
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Journal Title
J. Biochem
Volume: 155
Issue: 4
Pages: 235-241
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Indoxyl sulfate down-regulates SLCO4C1 transporter through up-regul ation of GATA32013
Author(s)
Akiyama Y, Kikuchi K, Saigusa D, Suzuki T, Takeuchi Y, Mishima E, Yamamoto Y, Ishida A, Sugawara D, Jinno D, Shima H, Toyohara T, Suzuki C, Souma T, Moriguchi T, Tomioka Y, Ito S, Abe T.
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Journal Title
PLoS One
Volume: 8
Issue: 7
Pages: e66518-e66518
DOI
Related Report
Peer Reviewed
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