Research Project
Grant-in-Aid for Young Scientists (B)
Protein S-nitrosylation is an important post-translational modification in a wide range of biological processes. However, direct detection of S-nitrosylated peptide using mass spectrometry is difficult because of instability and low abundance. The aim of this study is to develop a specific and sensitive method for analysis of protein S-nitrosylation using mass spectrometry. It was demonstrated that triphenylphosphine ester derivative is useful for the selective and efficient reduction of S-NO bonds compared with ascorbate and the water-soluble phosphine TXPTS is useful for the detection of S-nitrosylated peptide using mass spectrometry.
