| Project/Area Number |
25860024
|
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
|
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| Research Institution | Aichi Institute of Technology (2014)
Gifu Pharmaceutical University (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | DNA付加体 / 質量分析 / 高感度 / LC-MS / CE-MS / ガン / 網羅的 / DNA損傷体 |
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| Outline of Final Research Achievements |
Quantitation and identification of DNA adducts, which was induced by genotoxic compounds and their reactive metabolites, can be important for understanding the carcinogenic process and risk assessment. Sensitive methods have been used for the quantitation of DNA adducts because almost all of DNA adducts will be removed by inherent DNA repairing system, typically corresponding to a few adducts in million - billion normal 2’-deoxynucleosides. In this research, a more highly sensitive method than general one in liquid chromatography-mass spectrometry and capillary electrophoresis-mass spectrometry was studied. By optimizing each condition, we achieved an improvement in sensitivity by one order of magnitude in each system.
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