| Project/Area Number |
25860025
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
SASAI Yasushi 岐阜薬科大学, 薬学部, 准教授 (60336633)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | バイオセンシング / 表面機能化 / バイオコンジュゲート / 水晶振動子マイクロバランス / 生体分子固定化 / 表面・界面物性 / バイオセンサー / 生体分子固定化法 |
|---|
| Outline of Final Research Achievements |
In this study, the fabrication of the polymer-antibody conjugates with high substrate recognition ability in biosensing was investigated. Vinylmethylether-maleic acid copolymer (VEMAC) was used as a polymer for the conjugation and the VEMAC-antibody conjugates were synthesized on the gold electrode of quartz crystal oscillators for quartz crystal microbalance (QCM) biosensing. The VEMAC modification of gold electrode surface effectively suppressed the non-specific adsorption of proteins onto the surface. In the QCM experiments with quartz crystal oscillators modified with VEMAC-anti-C reactive protein (CRP) antibody conjugates, the results suggested that the anti-CRP antibody in the conjugate can quantitatively detect CRP with high sensitivity.
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