Regulation of intercellular crosstalk by induced ubiquitin ligase SSB

• Project/Area Number: 25860043
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Nagoya University
• Principal Investigator: FUMIHIKO Okumura 名古屋大学, 理学(系)研究科(研究院), 助教 (00507212)
• Co-Investigator(Renkei-kenkyūsha): 嘉村 巧 (40333455)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ユビキチン / ガン / タンパク質分解

Outline of Final Research Achievements

The aim is to identify the molecular mechanism which regulates intercellular crosstalk by SOCS box protein SSB.
We identified SSB-binding proteins. Given that SSB is a ubiquitin ligase, we checked the stability of SSB-binding proteins and found that one of the candidate accumulated by knockdown of SSB. The candidate has been reported as a tumor suppressor gene and also as a oncogene. We are currently studying the physiological function of the candidate protein.

Research Products

• [Journal Article] The nutrient stress-induced small GTPase Rab5 contributes to the activation of vesicle trafficking and vacuolar activity. (2014)
  • Author(s): Nakatsukasa K, Kanada A, Matsuzaki M, Byrne SD, Okumura F, Kamura T.
  • Journal Title: J Biol Chem. Volume: 289(30) Pages: 20970-20978
  • Peer Reviewed
• [Presentation] pVHLはFOBとHIF-αの分解を介してVHL病を制御する (2014)
  • Author(s): 1.奥村 文彦, 植松 桂司, 松崎 真理子, 平野 みえ, 奥村 晶子, 錦見 昭彦, 金森 正和, 執印 太郎, 福井 宣規, 中務 邦雄, 嘉村 巧
  • Organizer: 第37回 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-26
• [Presentation] Activation of PKR by ubiquitin-like molecule ISG15 modification down-regulates protein translation (2013)
  • Author(s): Fumihiko Okumura
  • Organizer: The Ubiquitin-Proteasome System: From Basic Mechanisms to Pathophysiological Roles
  • Place of Presentation: 札幌市
• [Presentation] Regulation of protein translation by ISG15-modified PKR (2013)
  • Author(s): 奥村 文彦，奥村 晶子，植松 桂司，畠山 鎮次，Dong-er Zhang，嘉村 巧
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸市