| Project/Area Number |
25860044
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
KATOH Yohei 京都大学, 薬学研究科(研究院), 助教 (90568172)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 一次繊毛 / 鞭毛 / 中心体 / 基底小体 / 低分子量GTPase / Rab Like / Rab-Like / バルデービードル症候群 / BBSome |
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| Outline of Final Research Achievements |
Rab-Like 2 (RabL2) protein belongs to the Ras superfamily of small GTPases. Although functions of RabL2 are unclear, the RabL2 gene is conserved in species that have motile cilia/flagella, and it was reported that the expression level of RabL2 is higher in ciliated cells than in non-ciliated ones. Moreover, male mice carrying a RabL2 mutation were reported to be sterile as a consequence of severely compromised sperm motility. Therefore, it is possible that RabL2 is implicated in functions of cilia/flagella.
In this study, we found that RabL2 binds to Cep19 in both GTP- and GDP-bound states, and the two proteins are co-localized on the centrosome and the basal body. Furthermore, we demonstrated that Cep19 recruits RabL2 to the centrosome. On the basis of these data, we propose a model in which RabL2 and Cep19 form a complex not only at the centrosome but also at the basal body, where they function together in ciliary motility.
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