Study on the role of protein arginine methyltransferase 5 in cardiac hypertrophy

• Project/Area Number: 25860052
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: University of Shizuoka
• Principal Investigator: Katanasaka Yasufumi 静岡県立大学, 薬学部, 講師 (00583973)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 心肥大 / 心不全 / アルギニンメチル化酵素 / 転写 / PRMT5 / MEP50 / p300/GATA4転写経路 / GATA4 / p300 / メチルトランスフェラーゼ

Outline of Final Research Achievements

Heart failure is one of the leading causes of death in industrialized countries, and cardiac hypertrophy is a risk factor for heart failure. We have demonstrated that PRMT5, a protein arginine methyltransferase, is involved in the promotion of cardiac hypertrophy and the development of heart failure. These results indicate that PRMT5 is a possible molecular target for heart failure therapy.

Research Products

• [Journal Article] Functional Analysis of GATA4 Complex, a Cardiac Hypertrophy-response Transcriptional Factor, Using a Proteomics Approach (2016)
  • Author(s): Sunagawa Y, Katanasaka Y, Wada H, Hasegawa K, Morimoto T.
  • Journal Title: YAKUGAKU ZASSHI Volume: 136 Issue: 2 Pages: 151-156
  • DOI: 10.1248/yakushi.15-00226-2
  • NAID: 130005121459
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2016-02-01
  • Peer Reviewed
• [Journal Article] Secreted frizzled-related protein 5 diminishes cardiac inflammation and protects the heart from ischemia-reperfusion injury. (2016)
  • Author(s): Nakamura K, Sano S, Fuster JJ, Kikuchi R, Shimizu I, Ohshima K, Katanasaka Y, Ouchi N, Walsh K.
  • Journal Title: J Biol Chem Volume: 291 Issue: 6 Pages: 2566-2575
  • DOI: 10.1074/jbc.m115.693937
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effects of Statins on Left Ventricular Diastolic Function in Patients with Dyslipidemia and Diastolic Dysfunction (Stat-LVDF Study) (2015)
  • Author(s): Morimoto T, Katanasaka Y, Sunagawa Y, Hirano S, Miyazaki Y, Funamoto M, Hojo Y, Suzuki H, Morimoto E, Ueno M, Shimatsu A, Satoh-Asahara N, Yamakage H, Wada H, Hasegawa K.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 38 Issue: 9 Pages: 1404-1409
  • DOI: 10.1248/bpb.b15-00126
  • NAID: 130005096994
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access
• [Presentation] PRMT5/MEP50 complex regulates p300/GATA4-induced cardiac hypertrophy (2016)
  • Author(s): Yusuke Miyazaki, Yasufumi Katanasaka, Yoichi Sunagawa, Hiromichi Wada, Koji Hasegawa and Tatsuya Morimoto
  • Organizer: 第80回日本循環器学会学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2016-03-20
• [Presentation] Protein Arginine Methyltransferase 5/Methylosome Protein 50 Complex Inhibits p300 Histone Acetyltransferase Activity and Hypertrophic Gene transcription in Cultured Cardiomyocytes (2015)
  • Author(s): Yusuke Miyazaki, Yasufumi Katanasaka, Yoichi Sunagawa, Hiromichi Wada, Koji Hasegawa and Tatsuya Morimoto
  • Organizer: AHA Scientific Sessions 2015
  • Place of Presentation: Orlando, USA
  • Year and Date: 2015-11-08
  • Int'l Joint Research
• [Presentation] PRMT5/MEP50メチル化複合体による心筋細胞肥大制御機構の解析 (2015)
  • Author(s): 刀坂泰史、中田淳也、宮崎雄輔、砂川陽一、鈴木秀敏、和田啓道、長谷川浩二、森本達也
  • Organizer: 第61回 日本薬学会東海支部大会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-07-04
• [Presentation] MEP50 RECRUITS PRMT5 TO p300 AND SUPRESSES p300/GATA4-INDUCED HYPERTROPHIC GENE TRANSCRIPTION (2015)
  • Author(s): Hiroki Wakabayashi, Yasufumi Katanasaka, Yusuke Miyazaki, Ryoga Sakurai, Yoichi Sunagawa, Hiromichi Wada, Koji Hasegawa, Tatsuya Morimoto
  • Organizer: Asian Pacific Society of Cardiology Congress 2015
  • Place of Presentation: UAE, Abu Dhabi
  • Year and Date: 2015-04-29
  • Int'l Joint Research
• [Presentation] Protein Arginine Methyltransferase 5 Inhibits cardiomyocyte Hypertrophy by methylating p300 in Cultured Cardiomyocytes (2015)
  • Author(s): Yuya Hojo, Yasufumi Katanasaka, Junya Nakada, Yoichi Sunagawa, Hidetoshi Suzuki, Hiromichi Wada, Koji Hasegawa, Tatsuya Morimoto
  • Organizer: Asian Pacific Society of Cardiology Congress 2015
  • Place of Presentation: UAE, Abu Dhabi
  • Year and Date: 2015-04-29
  • Int'l Joint Research
• [Presentation] PRMT5のメチル化活性がもたらす心肥大反応遺伝子転写抑制への影響 (2015)
  • Author(s): 若林弘樹、中田淳也、刀坂泰史、北條祐也、砂川陽一、鈴木秀敏、和田啓道、島津章、長谷川浩二、森本達也
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-27
• [Presentation] 心筋細胞内核内情報伝達機構の老性変化 (2015)
  • Author(s): 刀坂泰史
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-26
  • Invited
• [Presentation] 新規GATA4結合タンパク質MEP50の心肥大に対する機能解析 (2014)
  • Author(s): 櫻井涼賀、刀坂泰史、宮崎雄輔、中田淳也、砂川陽一、鈴木秀敏、和田啓道、長谷川浩二、森本達也
  • Organizer: 第5回 Molecular Cardiovascular Conference II
  • Place of Presentation: 神戸
  • Year and Date: 2014-09-05
• [Presentation] 心肥大反応における新規GATA4結合タンパク質PRMT5の機能解析 (2014)
  • Author(s): 北條祐也、刀坂泰史、中川由紀子、宮崎雄輔、中田淳也、砂川陽一、鈴木秀敏、和田啓道、長谷川浩二、森本達也
  • Organizer: 第5回 Molecular Cardiovascular Conference II
  • Place of Presentation: 神戸
  • Year and Date: 2014-09-05
• [Presentation] アルギニンメチル化酵素PRMT5による心肥大反応抑制機構の解析 (2014)
  • Author(s): 中田淳也，刀坂泰史，砂川陽一，鈴木秀敏，宮崎雄輔，北條祐也，和田啓道，長谷川浩二，森本達也
  • Organizer: 第51回日本臨床分子医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-11
• [Presentation] 新規GATA4結合タンパク質PRMT5の機能解析 (2014)
  • Author(s): 刀坂 泰史, 中川 由紀子, 宮崎 雄輔, 中田 淳也, 砂川 陽一, 鈴木 秀敏, 和田 啓道, 長谷川 浩二, 森本 達也
  • Organizer: 日本薬学会第134回年会
  • Place of Presentation: 熊本大学薬学部
• [Remarks] 静岡県立大学薬学部分子病態学分野
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/byoutai/