| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In the present study, we examined the roles of SM synthase (SMS) in HIV-1 Env-mediated membrane fusion using a cell-cell fusion assay with HIV-1 mimetics and their target cells. We employed reconstituted cells as target cells that stably express SMS1 or SMS2 in SMS-deficient cells. Fusion susceptibility was approximately five-fold higher in SMS2-expressing cells (not in SMS1-expressing cells) than in SMS-deficient cells. The enhancement of fusion susceptibility observed in SMS2-expressing cells was reversed and reduced by SMS2 knockdown. We also found that catalytically non-active SMS2 promoted membrane fusion susceptibility. Moreover, SMS2 co-localized and was constitutively associated with the HIV receptor/co-receptor complex in the plasma membrane. Taken together, our research provides insight into a novel function of SMS2: regulation of HIV-1 Env-mediated membrane fusion via actin rearrangement.
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