Sphingomyelin Synthase 2, but not Sphingomyelin Synthase 1, is Involved in HIV-1 Envelope-mediated Membrane Fusion
Project/Area Number |
25860055
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biological pharmacy
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Research Institution | Teikyo University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 脂質 / ヒト免疫不全ウィルス / HIV / スフィンゴ脂質 |
Outline of Final Research Achievements |
In the present study, we examined the roles of SM synthase (SMS) in HIV-1 Env-mediated membrane fusion using a cell-cell fusion assay with HIV-1 mimetics and their target cells. We employed reconstituted cells as target cells that stably express SMS1 or SMS2 in SMS-deficient cells. Fusion susceptibility was approximately five-fold higher in SMS2-expressing cells (not in SMS1-expressing cells) than in SMS-deficient cells. The enhancement of fusion susceptibility observed in SMS2-expressing cells was reversed and reduced by SMS2 knockdown. We also found that catalytically non-active SMS2 promoted membrane fusion susceptibility. Moreover, SMS2 co-localized and was constitutively associated with the HIV receptor/co-receptor complex in the plasma membrane. Taken together, our research provides insight into a novel function of SMS2: regulation of HIV-1 Env-mediated membrane fusion via actin rearrangement.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Insights into the Mechanism of Inhibition of CXCR4: Identification of Piperidinylethanamine Analogs as Anti-HIV-1 Inhibitors2015
Author(s)
Debananda Das, Kenji Maeda, Yasuhiro Hayashi, Navnath Gavande, Darshan, V., Desai, Simon, B. Chang, Arun, K., Ghosh, and Hiroaki Mitsuya
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Journal Title
Antimicrob. Agents Chemother.
Volume: 59
Issue: 4
Pages: 1895-1904
DOI
Related Report
Peer Reviewed
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