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Sulfation patterns of chondroitin sulfate regulate neural plasticity

Research Project

Project/Area Number 25860057
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biological pharmacy
Research InstitutionNagoya University

Principal Investigator

Miyata Shinji  名古屋大学, 生物機能開発利用研究センター, 助教 (60533792)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsプロテオグリカン / コンドロイチン硫酸 / ペリニューロナルネット / 神経可塑性 / 糖鎖生物学
Outline of Final Research Achievements

Perineuronal nets (PNNs) are lattice-like extracellular matrix structures composed of chondroitin sulfate proteoglycans (CSPGs). It has been shown that sulfation patterns of chondroitin sulfate (CS) chains on CSPGs influenced neural plasticity. However, the mechanism of PNN formation regulated by CS sulfation remains unknown. In this study, I found that overexpression of chondroitin 6-sulfotransferase-1 (C6ST-1) selectively decreased aggrecan, a major CSPG in PNNs, in the aged brain without affecting other PNN components. Overexpression of 6-sulfation primarily impaired accumulation of aggrecan in PNNs. Increased 6-sulfation accelerated proteolysis of aggrecan by a disintegrin and metalloproteinase domain with thrombospondin motif protease. These results indicate that sulfation patterns of CS chains on aggrecan influenced the stability of the CSPG, thereby regulating formation of PNNs and neural plasticity.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (6 results) (of which Invited: 2 results)

  • [Journal Article] Chondroitin 6-sulfation regulates perineuronal net formation by controlling the stability of aggrecan.2016

    • Author(s)
      Miyata, S., and Kitagawa, H.
    • Journal Title

      Neural Plast.

      Volume: 2016 Pages: 1305801-1305801

    • DOI

      10.1155/2016/1305801

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Chondroitin sulfate and neuronal disorders.2016

    • Author(s)
      Miyata, S., and Kitagawa, H.
    • Journal Title

      Front. Biosci.

      Volume: 21 Issue: 7 Pages: 1330-1340

    • DOI

      10.2741/4460

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Mechanisms for modulation of neural plasticity and axon regeneration by chondroitin sulfate.2015

    • Author(s)
      Miyata, S., and Kitagawa, H.
    • Journal Title

      J. Biochem.

      Volume: 157 Issue: 1 Pages: 13-22

    • DOI

      10.1093/jb/mvu067

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] N-acetylgalactosaminyl-transferase-1 inhibits recovery from neural injury.2013

    • Author(s)
      Kosei Takeuchi, Nozomu Yoshioka, Susumu Higa Onaga, Yumi Watanabe, et. al.
    • Journal Title

      Nature Communications

      Volume: 4 Issue: 1 Pages: 2740-2740

    • DOI

      10.1038/ncomms3740

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Sulfation patterns of chondroitin sulfate regulate neural development and plasticity2014

    • Author(s)
      Shinji Miyata, Yukio Komatsu, Yumiko Yoshimura, Choji Taya and Hiroshi Kitagawa
    • Organizer
      SFG & JSCR Joint Annual Meeting
    • Place of Presentation
      ハワイ
    • Year and Date
      2014-10-16 – 2014-10-19
    • Related Report
      2014 Research-status Report
  • [Presentation] コンドロイチン硫酸による神経細胞の移動と極性の制御2014

    • Author(s)
      宮田真路, 北川裕之
    • Organizer
      第33回日本糖質学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-08-10 – 2014-08-12
    • Related Report
      2014 Research-status Report
  • [Presentation] コンドロイチン硫酸の構造が決める神経の発生と可塑性2014

    • Author(s)
      宮田真路
    • Organizer
      第146回薬学談話会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-07-09
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] コンドロイチン硫酸による大脳皮質形成と神経可塑性の制御2014

    • Author(s)
      宮田真路, 北川裕之
    • Organizer
      第66回日本細胞生物学会大会
    • Place of Presentation
      奈良
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Roles of chondroitin sulfate in neural development and plasticity2013

    • Author(s)
      Shinji Miyata and Hiroshi Kitagawa
    • Organizer
      Neuro2013
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] コンドロイチン硫酸の硫酸化パターンによる眼優位性可塑性の制御2013

    • Author(s)
      宮田真路、小松由紀夫、吉村由美子、多屋長治、北川裕之
    • Organizer
      第32回日本糖質学会年会
    • Place of Presentation
      大阪国際交流センター
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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