Mechanism of nucleotide accumulation and release in blood cells

• Project/Area Number: 25860062
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pharmacology in pharmacy
• Research Institution: Okayama University
• Principal Investigator: HIASA Miki 岡山大学, 医歯(薬)学総合研究科, 助教 (30587720)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 血小板 / ヌクレオチド / ATP / VNUT / 化学伝達 / 好中球 / プリン作動性化学伝達

Outline of Final Research Achievements

The mechanisms of nucleotide accumulation and release in blood cells are unclear. In this study, we analyzed vesicular nucleotide transporter (VNUT) function and localization in platelet. VNUT was localized in dense granules and play crucial roles in nucleotide accumulation and release in platelets. We also found an effective VNUT inhibitor, glyoxylate.

Research Products

• [Journal Article] Identification of a mammalian vesicular polyamine transporter (2014)
  • Author(s): Hiasa M., Miyaji T., Haruna Y., Takeuchi T., Harada Y., Moriyama S., Yamamoto A., Omote H., Moriyama Y.
  • Journal Title: Sci. Rep. Volume: 4 Issue: 1 Pages: 6836-6836
  • DOI: 10.1038/srep06836
  • NAID: 120005477132
  • Peer Reviewed / Open Access
• [Journal Article] Impairment of vesicular ATP release affects glucose metabolism and increases insulin sensitivity (2014)
  • Author(s): Sakamoto S., Miyaji T.,, Hiasa M., Ichikawa R., Iwatsuki K., Shibata A., Uneyama H., Takayanagi R., Yamamoto A., Omote H., Nomura M., Moriyama Y.
  • Journal Title: Sci. Rep. Volume: 4 Issue: 1 Pages: 6689-6689
  • DOI: 10.1038/srep06689
  • Peer Reviewed / Open Access
• [Journal Article] Essential role of vesicular nucleotide transporter in vesicular storage and release of nucleotides in platelets (2014)
  • Author(s): Miki Hiasa, Natsuko Togawa, Takaaki Miyaji, Hiroshi Omote, Akitsugu Yamamoto, Yoshinori Moriyama
  • Journal Title: Physiological Reports Volume: 2 Issue: 6 Pages: e12034-e12034
  • DOI: 10.14814/phy2.12034
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Immunological Identification of Vesicular Nucleotide Transporter in Intestinal L Cells (2014)
  • Author(s): Harada Y, Hiasa M.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 37 Issue: 7 Pages: 1090-1095
  • DOI: 10.1248/bpb.b14-00275
  • NAID: 130004057432
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Suppression of increased blood glucose levels in mice by Awa-ban tea following oral administration of mono- and disaccharides. (2014)
  • Author(s): Hiasa M, Kurokawa M, Akita H, Harada M, Niki K, Ohta K, Shoji M, Echigo N, Kuzuhara T.
  • Journal Title: Journal of Functional Foods Volume: 8 Pages: 188-192
  • DOI: 10.1016/j.jff.2014.03.012
  • Peer Reviewed / Open Access
• [Journal Article] Identification and purification of resorcinol, an antioxidant specific to Awa-ban (pickled and anaerobically fermented) tea. (2013)
  • Author(s): Hiasa M, Kurokawa M, Ohta K, Esumi T, Akita H, Niki K, Yagi Y, Echigo N, Hatakeyama D, Kuzuhara T.
  • Journal Title: Food Research International Volume: (in press) Issue: 1 Pages: 72-80
  • DOI: 10.1016/j.foodres.2013.05.036
  • Peer Reviewed
• [Journal Article] Vesicular nucleotide transporter regulates the nucleotide content in airway epithelial mucin granules (2013)
  • Author(s): Sesma J, Kreda SM, Okada SF, Heu sden C, Moussa L, Jones LC, O'Nea I WK, Togawa N, Hiasa M, Moriyama Y, Lazarowski ER
  • Journal Title: American Journal of Physiology Cell Physiology Volume: 304(10) Issue: 10 Pages: C976-C984
  • DOI: 10.1152/ajpcell.00371.2012
  • Peer Reviewed
• [Presentation] 新規小胞型ポリアミントランスポーターの発現と機能解析 (2015)
  • Author(s): 日浅未来，宮地孝明，竹内智也, 表弘志，森山芳則
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-26
• [Presentation] Physiological function of vesicular nucleotide transporter (VNUT) in purinergic cells. (2015)
  • Author(s): Miki Hiasa
  • Organizer: 第88回日本薬理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-03-18
  • Invited
• [Presentation] 中枢における新規小胞型ポリアミントランスポーターの発現および機能解析 (2014)
  • Author(s): 日浅未来，宮地孝明，竹内智也, 表弘志，森山芳則
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-16
• [Presentation] Vesicular nucleotide transporter (VNUT) in platelets. (2014)
  • Author(s): Miki Hiasa
  • Organizer: Purines 2014
  • Place of Presentation: Bonn, German
  • Year and Date: 2014-07-24
  • Invited
• [Presentation] 神経細胞における小胞型ヌクレオチドトランスポーターの局在と機能
  • Author(s): 日浅未来 et al.
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜