Elucidation of the biosynthetic mechanism that leads to the diversity of the tetracyclic skeleton of diterpenes

• Project/Area Number: 25860076
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Natural medicines
• Research Institution: University of Toyama
• Principal Investigator: YAMAMURA Yoshimi 富山大学, 大学院医学薬学研究部(薬学), 助教 (30464027)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 植物二次代謝 / ジテルペン / チトクロームP450 / 環化酵素 / 有用物質生産 / エリシター / 二次代謝 / 物質生産

Outline of Final Research Achievements

In this study, SdCYP5, SdCYP6, SdCYP7 and SdCYP8, were isolated as elicitor inducible cytochrome P450 (P450) candidate genes, are classified in the CYP71 family. CYP71 family is involved in a variety of secondary metabolism in higher plants. The gene expression of SdCYP5 and SdCYP8 had a close relationship with SDB biosynthesis activity in Scoparia leaves. In addition, we also successfully isolated a set of diterpene biosynthetic enzyme genes, an ent-kaurene oxidase (SdKO1 and SdKO2, CYP701A family) and ent-kaurenoic acid oxidase (SdKAO1 and SdKAO2, CYP88A family) genes.

Research Products

• [Journal Article] Transcriptional activation of a geranylgeranyl diphosphate synthase gene, GGPPS2, isolated from Scoparia dulcis by the treatment with methyl jasmonate and yeast extract.
  • Author(s): Yamamura, Y., Mizuguchi, Y., Taura, F. & Kurosaki, F
  • Journal Title: Journal of Natural Medicines Volume: 68 Issue: 4 Pages: 748-753
  • DOI: 10.1007/s11418-014-0855-7
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Cloning, E. coli expression, and characterization of cinnamic acid 4-hydroxylase from Scoparia dulcis. (2014)
  • Author(s): Yamamura Y, Mabuchi A, Kurosaki F
  • Organizer: 12th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2014-09-24 – 2014-09-28
• [Presentation] Functional characterization of an inducible NADPH:cytochrome P450 reductase from a tropical medicinal plant Scoparia dulcis. (2014)
  • Author(s): Yamamura Y, Mabuchi A, Kurosaki F
  • Organizer: Plant Biology Meeting (Annual Scientific Meeting of the American Society of Plant Biologists)
  • Place of Presentation: Portland, U.S.A.
  • Year and Date: 2014-06-12 – 2014-06-16
• [Presentation] Molecular cloning and functional characterization of an NADPH: cytochrome P450 reductase from a tropical medicinal plant Scoparia dulcis. (2013)
  • Author(s): Yoshimi Yamamura, Ayaka Mabuchi and Fumiya Kurosaki
  • Organizer: 61st International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA)
  • Place of Presentation: Munster, Germany
• [Presentation] 薬用植物スコパリア由来のNADPH：シトクロームP450 還元酵素の単離と解析 (2013)
  • Author(s): 馬渕彩香、山村良美、黒崎文也
  • Organizer: 第31回日本植物細胞分子生物学会（札幌）大会
  • Place of Presentation: 札幌