Establishment of a New Method for Huperzine A Production using Plant Endophytic Fungi
Project/Area Number |
25860080
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Natural medicines
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Research Institution | Nagoya City University (2015) Nihon University (2013-2014) |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | huperzine A / アルツハイマー病 / 植物内生糸状菌 / Phoma sp. / Lycopodium alkaloid / serralongamine / マクロファージ泡沫化阻害活性 / 生合成遺伝子 / serralongamine B / 生物合成 / serralongamine A |
Outline of Final Research Achievements |
We discovered a fungal strain Lsl3 from Lycopodium serratum var. longipetiolatum produced huperzine A, an anti-Alzheimer’s disease drug candidate from the traditional Chinese medicine Qian Ceng Ta (Huperzia serrata). The strain Lsl3 was classified to the genus Phoma based on molecular phylogenetic analysis. Also we isolated four new alkaloids, serralongamines A-D, from L. serratum var. longipetiolatum and elucidated their stuructures on the basis of spectroscopic data. Serralongamines B and D exhibited the inhibitory activity against foam cell formation in human macrophages, one of characteristic features of early atherosclerotic lesions.
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Report
(4 results)
Research Products
(17 results)