| Project/Area Number |
25860084
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Natural medicines
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| Research Institution | Hoshi University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | キョウチクトウ科 / アルカロイド / Ａｐｏｃｙｎａｃｅａｅ / インドールアルカロイド / Tabernaemontana / Voacanga / voacalgine / grandifolia |
|---|
| Outline of Final Research Achievements |
Twelve new indole alkaloids including three novel skeletons were isolated from Voacanga grandifolia and Tabernaemontana corymbosa. The novel alkaloid, voacalgine A showed moderate cell growth inhibitory activities against HL-60 and HCT116 cells. 9-Methoxyellipticine (9-Mep) isolated from Tabernaemontana phymata specificaly inhibits C/EBPβ expression of murine macrophage cell line, RAW264.7 and murine pre-adipocyte cell line, MC3T3-G2/PA6. 9-Mep was found to inhibit C/EBPβ expression of both mRNA and protein level. Inhibition of RAW264.7 cells C/EBPβ expression led to attenuation of TNF-α, COX-2, and MCP-1 expression suggesting anti inflammatory activity. On the other hand, inhibition of MC3T3-G2/PA6 cells C/EBPβ expression interupted MC3T3-G2/PA6 differentation into adipocyte.
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