Development of therapeutic and diagnostic methods for gastric MALT lymphoma by infection with Helicobacter heilmannii

• Project/Area Number: 25860099
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental and hygienic pharmacy
• Research Institution: Kitasato University
• Principal Investigator: TAKAHASHI Tetsufumi 北里大学, 薬学部, 助教 (40449004)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: Helicobacter / heilmannii / MALTリンパ腫 / ヘリコバクター / MALT / ハイルマニイ

Outline of Final Research Achievements

In this study, to determine the malignant factor of gastric MALT lymphoma by infection with H. heilmannii, gene expression profiling analysis in the serum and liver of chronic infected mice was performed using microarray method. The results revealed that 15 genes, which were upregulated in both serum and liver, were determined. In addition, we investigated the therapeutic effect of mutant CTLA4-Ig, which is strong blocker of CD86-CD28 interaction. Administration of mutant CTLA4-Ig antigen attenuated the gastric MALT lymphoma formation and the mRNA expression of the marker gene of the gastric MALT lymphoma component B cells.

Research Products

• [Journal Article] Development of a PCR method for the detection of Helicobacter suis in gastric biopsy specimens. (2014)
  • Author(s): Matsui H, Takahashi T, Yamagata-Murayama S, Uchiyama I, Yamaguchi K, Shigenobu S, Matsumoto T, Kawakubo M, Ota H, Osaki T, Kamiya S, Takahashi S, Nakamura S, Nakamura M
  • Journal Title: Helicobacter Volume: in press Issue: 4 Pages: 260-271
  • DOI: 10.1111/hel.12127
  • Peer Reviewed
• [Journal Article] Alteration of angiogenesis in Helicobacter-heilmannii-induced MALT lymphoma: interaction with c-Met and HGF. (2014)
  • Author(s): Nakamura, M., Takahashi, T., Matsui, H., Baniwa, Y., Takahashi, S., Murayama, S.Y., Serizawa, H., Suzuki, H., Hibi, T.
  • Journal Title: J. Gastroenterol. Hepatol. Volume: 29 Issue: S4 Pages: 70-76
  • DOI: 10.1111/jgh.12776
  • Peer Reviewed / Open Access
• [Journal Article] New pharmaceutical treatment of gastric MALT lymphoma: anti-angiogenesis treatment using VEGF receptor antibodies and celecoxib. (2014)
  • Author(s): Nakamura M, Takahashi T, Matsui H, Takahashi S, Murayama SY, Suzuki H, Tsuchimoto K
  • Journal Title: Curr Pharm Des. Volume: 20 Pages: 1097-103
  • Peer Reviewed
• [Presentation] Helicobacter heilmannii感染による胃MALTリンパ腫発症機構の解析と新規治療法の探索 (2014)
  • Author(s): 高橋哲史、中村正彦、土本寛二
  • Organizer: 第２０回日本ヘリコバクター学会学術集会
  • Place of Presentation: ステーションコンファレンス 東京
  • Year and Date: 2014-06-28
• [Presentation] 胃 MALTリンパ腫形成における substance P, NK1Rの関与 (2013)
  • Author(s): 中村 正彦, 高橋 哲史, 松井 英則, 土本 寛二
  • Organizer: 第66回日本自律神経学会総会
  • Place of Presentation: 愛知県産業労働センターウインクあいち