| Project/Area Number |
25860101
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Environmental and hygienic pharmacy
|
|---|
| Research Institution | Showa University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 危険ドラッグ / アプタマー / スクリーニング / SELEX / JWH-018 / MDA |
|---|
| Outline of Final Research Achievements |
Wide spread use of recreational drugs has become a serious problem in our society. Although novel psychoactive drugs are being regulated comprehensively by Japanese government recently, current GC or LC-MS-based analytical method, may take an excessive burden for the regulatory authority. Thus, it is urgently necessary to develop a test useful for newly marketed drugs. DNA aptamer can detect a low molecular compound with high affinity and be produced stable in a short time compared with antibodies. The present study is aiming at applying DNA aptamer to a test of abused drugs. DNA aptamers that recognizes synthetic cannabinoids are generated by systematic evolution of ligands by exponential enrichment (SELEX) methods. After ten rounds of SELEX, some DNA aptamers were successfully obtained and analyzed those sequences. On-site testing for drugs of abuse would be developed by using these DNA aptamers.
|
