| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The intrarenal dopaminergic system is likely responsible for regulating over 50% of net renal salt and water excretion when salt intake increases. Although dopamine synthesis is localized at proximal tubular cells, the molecular mechanism underlying dopamine secretion into urine remains unknown. We analyzed transport properties of dopamine by human and mouse multidrug and toxin extrusion (MATE) and measured urinary dopamine and sodium in wild-type and Mate1 null mice. Dopamine uptake by MATE exhibits saturable kinetics. The amount of urinary dopamine and sodium was decreased in Mate1 null mice. In conclusion, MATE plays a critical role in dopamine secretion into tubular lumen and promotes natriuresis.
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