| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Outline of Final Research Achievements |
We found several effective small-molecule compounds on inducing differentiation of human iPS cells to enterocytes and gain of pharmacokinetic function. The enterocyte-like cells differentiated by using these compounds expressed intestinal markers and exhibited drug-metabolizing enzyme activities, CYP3A4 inducibility, and active peptide transport. We demonstrated to culture the intestinal stem cell-like cells with differentiation capability for about 1 month and to cryopreserve the cells. These results would provided useful information for application of iPS cell-derived enterocytes to drug development studies.
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