Differentiation of human iPS cells to pharmacokinetically functional enterocytes
| Project/Area Number |
25860120
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Nagoya City University |
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Principal Investigator |
IWAO Takahiro 名古屋市立大学, 薬学研究科(研究院), 講師 (50581740)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | iPS細胞 / 小腸 / 分化誘導 / 薬物動態 |
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| Outline of Final Research Achievements |
We found several effective small-molecule compounds on inducing differentiation of human iPS cells to enterocytes and gain of pharmacokinetic function. The enterocyte-like cells differentiated by using these compounds expressed intestinal markers and exhibited drug-metabolizing enzyme activities, CYP3A4 inducibility, and active peptide transport. We demonstrated to culture the intestinal stem cell-like cells with differentiation capability for about 1 month and to cryopreserve the cells. These results would provided useful information for application of iPS cell-derived enterocytes to drug development studies.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] An Efficient Method for Differentiation of Human Induced Pluripotent Stem Cells into Hepatocyte-like Cells Retaining Drug Metabolizing Activity2014
Author(s)
Kondo Y, Iwao T, Nakamura K, Sasaki T, Takahashi S, Kamada N, Matsubara T, Gonzalez FJ, Akutsu H, Miyagawa Y, Okita H, Kiyokawa N, Toyoda M, Umezawa A, Nagata K, Matsunaga T, Ohmori S
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Journal Title
Drug Metabolism and Pharmacokinetics
Volume: 29
Issue: 3
Pages: 237-243
DOI
NAID
ISSN
1347-4367, 1880-0920
Related Report
Peer Reviewed
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