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Functional analysis of novel polarity-related genes for hippocampal neuronal formation

Research Project

Project/Area Number 25860141
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General anatomy (including histology/embryology)
Research InstitutionUniversity of Yamanashi (2015)
Osaka University (2013-2014)

Principal Investigator

IWANO Tomohiko  山梨大学, 総合研究部, 助教 (10442930)

Research Collaborator HARADA Akihiro  大阪大学, 医学研究科, 教授 (40251441)
YOSHIMURA Shin-ichiro  大阪大学, 医学研究科, 助教 (60584521)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsノックアウトマウス / 極性輸送 / 神経細胞 / 酵母2-ハイブリッド解析 / インタラクトーム
Outline of Final Research Achievements

To know the functional relationship of novel polarity-related genes for hippocampal neuronal formation, I analyzed the genes which involved in the polarity formation using C. elegans. RNAi analysis. I performed the yeast two-hybrid system to identify the physical relationships of the candidate genes. However, I couldn't identify the significant result of their physical relationships. Therefore, I investigated the gene Rab1, whose knockdown showed significant abnormality in polarized epithelial cells of C.elegans. I analyzed the conditional knockout mice for Rab1a using Nestin-Cre, but can't see any significant abnormality in the behavior and the brain formation. This might come from the redundancy of Rab1b, which is a gene in Rab1 family. Therefore, I must analyze the Rab1a and 1b double knockout mice in the future research.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2015 2014 2013

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (1 results)

  • [Journal Article] Prox1 Regulates the Subtype-Specific Development of Caudal Ganglionic Eminence-Derived GABAergic Cortical Interneurons.2015

    • Author(s)
      Miyoshi G, Young A, Petros T, Karayannis T, McKenzie Chang M, Lavado A, Iwano T, Nakajima M, Taniguchi H, Huang ZJ, Heintz N, Oliver G, Matsuzaki F, Machold RP, Fishell G.
    • Journal Title

      Journal of Neuroscience

      Volume: 35 Issue: 37 Pages: 12869-12889

    • DOI

      10.1523/jneurosci.1164-15.2015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Functional redundancy of protein kinase D1 and protein kinase D2 in neuronal polarity.2015

    • Author(s)
      Avriyanti E, Atik N, Kunii M, Furumoto N, Iwano T, Yoshimura S, Harada R, Harada A.
    • Journal Title

      Neuroscience Research

      Volume: 95 Pages: 12-20

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Functional redundancy of protein kinase D1 and protein kinase D2 in neuronal polarity.2015

    • Author(s)
      Avriyanti E, Atik N, Kunii M, Furumoto N, Iwano T, Yoshimura S, Harada R, Harada A.
    • Journal Title

      Neuroscience Research

      Volume: 95 Pages: 12-20

    • DOI

      10.1016/j.neures.2015.01.007

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Rab11a is required for apical protein localisation in the intestine.2014

    • Author(s)
      Sobajima T, Yoshimura S, Iwano T, Kunii M, Watanabe M, Atik N, Mushiake S, Morii E, Koyama Y, Miyoshi E, Harada A
    • Journal Title

      Biol Open

      Volume: 4 Issue: 1 Pages: 86-94

    • DOI

      10.1242/bio.20148532

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis2014

    • Author(s)
      Takashi Sato, Tomohiko Iwano, Masataka Kunii, Shinji Matsuda, Rumiko Mizuguchi, Yongwook Jung, Haruo Hagiwara, Yoshihiro Yoshihara, Michisuke Yuzaki, Reiko Harada and Akihiro Harada
    • Journal Title

      Journal of Cell Science

      Volume: 127 Pages: 422-431

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rab8a and Rab8b are essential for multiple apical transport pathways but insufficient for ciliogenesis.2013

    • Author(s)
      Sato T, Iwano T, Kunii M, Matsuda S, Mizoguchi R, Jung Y, Hagiwara H, Yoshihara Y, Yuzaki M, Harada R, Harada A.
    • Journal Title

      J Cell Sci.

      Volume: 15;127(Pt 2) Pages: 422-31

    • DOI

      10.1242/jcs.136903

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis2014

    • Author(s)
      岩野智彦
    • Organizer
      日本解剖学会総会
    • Place of Presentation
      自治医科大学
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2023-03-16  

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