Functional analysis of novel polarity-related genes for hippocampal neuronal formation

• Project/Area Number: 25860141
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: University of Yamanashi (2015); Osaka University (2013-2014)
• Principal Investigator: IWANO Tomohiko 山梨大学, 総合研究部, 助教 (10442930)
• Research Collaborator: HARADA Akihiro 大阪大学, 医学研究科, 教授 (40251441) ; YOSHIMURA Shin-ichiro 大阪大学, 医学研究科, 助教 (60584521)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ノックアウトマウス / 極性輸送 / 神経細胞 / 酵母2-ハイブリッド解析 / インタラクトーム

Outline of Final Research Achievements

To know the functional relationship of novel polarity-related genes for hippocampal neuronal formation, I analyzed the genes which involved in the polarity formation using C. elegans. RNAi analysis. I performed the yeast two-hybrid system to identify the physical relationships of the candidate genes. However, I couldn't identify the significant result of their physical relationships. Therefore, I investigated the gene Rab1, whose knockdown showed significant abnormality in polarized epithelial cells of C.elegans. I analyzed the conditional knockout mice for Rab1a using Nestin-Cre, but can't see any significant abnormality in the behavior and the brain formation. This might come from the redundancy of Rab1b, which is a gene in Rab1 family. Therefore, I must analyze the Rab1a and 1b double knockout mice in the future research.

Research Products

• [Journal Article] Prox1 Regulates the Subtype-Specific Development of Caudal Ganglionic Eminence-Derived GABAergic Cortical Interneurons. (2015)
  • Author(s): Miyoshi G, Young A, Petros T, Karayannis T, McKenzie Chang M, Lavado A, Iwano T, Nakajima M, Taniguchi H, Huang ZJ, Heintz N, Oliver G, Matsuzaki F, Machold RP, Fishell G.
  • Journal Title: Journal of Neuroscience Volume: 35 Issue: 37 Pages: 12869-12889
  • DOI: 10.1523/jneurosci.1164-15.2015
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Functional redundancy of protein kinase D1 and protein kinase D2 in neuronal polarity. (2015)
  • Author(s): Avriyanti E, Atik N, Kunii M, Furumoto N, Iwano T, Yoshimura S, Harada R, Harada A.
  • Journal Title: Neuroscience Research Volume: 95 Pages: 12-20
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Functional redundancy of protein kinase D1 and protein kinase D2 in neuronal polarity. (2015)
  • Author(s): Avriyanti E, Atik N, Kunii M, Furumoto N, Iwano T, Yoshimura S, Harada R, Harada A.
  • Journal Title: Neuroscience Research Volume: 95 Pages: 12-20
  • DOI: 10.1016/j.neures.2015.01.007
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Rab11a is required for apical protein localisation in the intestine. (2014)
  • Author(s): Sobajima T, Yoshimura S, Iwano T, Kunii M, Watanabe M, Atik N, Mushiake S, Morii E, Koyama Y, Miyoshi E, Harada A
  • Journal Title: Biol Open Volume: 4 Issue: 1 Pages: 86-94
  • DOI: 10.1242/bio.20148532
  • Peer Reviewed / Open Access
• [Journal Article] Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis (2014)
  • Author(s): Takashi Sato, Tomohiko Iwano, Masataka Kunii, Shinji Matsuda, Rumiko Mizuguchi, Yongwook Jung, Haruo Hagiwara, Yoshihiro Yoshihara, Michisuke Yuzaki, Reiko Harada and Akihiro Harada
  • Journal Title: Journal of Cell Science Volume: 127 Pages: 422-431
  • Peer Reviewed
• [Journal Article] Rab8a and Rab8b are essential for multiple apical transport pathways but insufficient for ciliogenesis. (2013)
  • Author(s): Sato T, Iwano T, Kunii M, Matsuda S, Mizoguchi R, Jung Y, Hagiwara H, Yoshihara Y, Yuzaki M, Harada R, Harada A.
  • Journal Title: J Cell Sci. Volume: 15;127(Pt 2) Pages: 422-31
  • DOI: 10.1242/jcs.136903
  • Peer Reviewed / Open Access
• [Presentation] Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis (2014)
  • Author(s): 岩野智彦
  • Organizer: 日本解剖学会総会
  • Place of Presentation: 自治医科大学